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Journal of Bacteriology, September 1998, p. 4750-4752, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Gene Transfer between Related Bacteria by Electrotransformation: Mapping Salmonella typhi Genes in Salmonella typhimurium

Cecilia S. Toro,1 Guido C. Mora,1 and Nara Figueroa-Bossi2,*

Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biologicas, Pontificia Universidad Católica de Chile,1 and Centre de Génétique Moléculaire du CNRS, 91198 Gif-sur-Yvette Cedex, France2

Received 17 April 1998/Accepted 6 July 1998

Transfer of newly isolated mutations into a fresh background is an essential step of genetic analysis and strain construction. Gene transfer is hampered in Salmonella typhi and in other pathogenic bacteria by the lack of a generalized transduction system. We show here that this problem can be partially circumvented by using electrotransformation as a means for delivering S. typhi DNA into suitable S. typhi or Salmonella typhimurium recipients. Transferred DNA can recombine with the homologous region in the host chromosome. In one application of the method, mutations isolated in S. typhi were genetically mapped in S. typhimurium.

* Corresponding author. Mailing address: Centre de Génétique Moléculaire du CNRS, 91198 Gif-sur-Yvette Cédex, France. Phone: 33-1-69-82-31-79. Fax: 33-1-69 82 32 30; E-mail: figueroa{at}cgm.cnrs-gif.fr.

Journal of Bacteriology, September 1998, p. 4750-4752, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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