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Journal of Bacteriology, September 1998, p. 4775-4780, Vol. 180, No. 18
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Mutations in Salmonella Pathogenicity Island 2 (SPI2) Genes Affecting Transcription of SPI1 Genes and Resistance to Antimicrobial Agents

Jörg Deiwick,1 Thomas Nikolaus,1 Jaqueline E. Shea,2 Colin Gleeson,2 David W. Holden,2 and Michael Hensel1,*

Lehrstuhl für Bakteriologie, Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie der Ludwig-Maximillians-Universität München, Munich, Germany,1 and Department of Infectious Diseases, Imperial College of Medicine, Hammersmith Hospital, London, United Kingdom2

Received 20 January 1998/Accepted 11 July 1998

The Salmonella typhimurium genome contains two pathogenicity islands (SPI) with genes encoding type III secretion systems for virulence proteins. SPI1 is required for the penetration of the epithelial layer of the intestine. SPI2 is important for the subsequent proliferation of bacteria in the spleens of infected hosts. Although most mutations in SPI2 lead to a strong reduction of virulence, they have different effects in vitro, with some mutants having significantly increased sensitivity to gentamicin and the antibacterial peptide polymyxin B. Previously we showed that certain mutations in SPI2 affect the ability of S. typhimurium to secrete SPI1 effector proteins and to invade cultured eukaryotic cells. In this study, we show that these SPI2 mutations affect the expression of the SPI1 invasion genes. Analysis of reporter fusions to various SPI1 genes reveals highly reduced expression of sipC, prgK, and hilA, the transcriptional activator of SPI1 genes. These observations indicate that the expression of one type III secretion system can be influenced dramatically by mutations in genes encoding a second type III secretion system in the same cell.

* Corresponding author. Mailing address: Lehrstuhl für Bakteriologie, Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Pettenkofer Str. 9a, D-80336 Munich, Germany. Phone: 49 (0)89 5160 5248. Fax: 49 (0)89 5160 5223. E-mail: hensel{at}m3401.mpk.med.uni-muenchen.de.

Journal of Bacteriology, September 1998, p. 4775-4780, Vol. 180, No. 18
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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