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Journal of Bacteriology, September 1998, p. 4834-4842, Vol. 180, No. 18
Laboratoire de Microbiologie et
Génétique Moléculaire du CNRS, Université
Paul Sabatier, 31062 Toulouse, France
Received 6 May 1998/Accepted 13 July 1998
Comparative analysis of chromosomal macrorestriction polymorphism
of the two closely related Lactococcus lactis subsp.
cremoris strains MG1363 and NCDO763 revealed the presence
of a large inversion covering half of the genome. To determine what
kind of genetic element could be implicated in this rearrangement, the
two inversion junctions of MG1363 and NCDO763 chromosomes were cloned
and characterized. Nucleotide sequence analysis showed the presence of
one copy of the lactococcal IS905 element in each junction.
Each copy of this element contained the same nucleotide mutation that
inactivates the putative transposase. Comparison of the sequences
surrounding the insertion sequence demonstrated that the large
inversion arose from a single-step homologous recombination event
between the two defective copies of the IS905 element. The
large inversion presumably conferred no selective disadvantage on
strain NCDO763 because this rearrangement did not alter the
oriC-terC symmetry of the chromosome and the local genetic
environment.
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
A Natural Large Chromosomal Inversion in
Lactococcus lactis Is Mediated by Homologous Recombination
between Two Insertion Sequences
*
Corresponding author. Mailing address: LMGM du CNRS,
Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse
Cedex, France. Phone: (33) 561 33 58 25. Fax: (33) 561 33 58 86. E-mail: ritzenth{at}ibgc.biotoul.fr.
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