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Journal of Bacteriology, September 1998, p. 4834-4842, Vol. 180, No. 18
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Natural Large Chromosomal Inversion in Lactococcus lactis Is Mediated by Homologous Recombination between Two Insertion Sequences

Marie-Line Daveran-Mingot, Nathalie Campo, Paul Ritzenthaler,* and Pascal Le Bourgeois

Laboratoire de Microbiologie et Génétique Moléculaire du CNRS, Université Paul Sabatier, 31062 Toulouse, France

Received 6 May 1998/Accepted 13 July 1998

Comparative analysis of chromosomal macrorestriction polymorphism of the two closely related Lactococcus lactis subsp. cremoris strains MG1363 and NCDO763 revealed the presence of a large inversion covering half of the genome. To determine what kind of genetic element could be implicated in this rearrangement, the two inversion junctions of MG1363 and NCDO763 chromosomes were cloned and characterized. Nucleotide sequence analysis showed the presence of one copy of the lactococcal IS905 element in each junction. Each copy of this element contained the same nucleotide mutation that inactivates the putative transposase. Comparison of the sequences surrounding the insertion sequence demonstrated that the large inversion arose from a single-step homologous recombination event between the two defective copies of the IS905 element. The large inversion presumably conferred no selective disadvantage on strain NCDO763 because this rearrangement did not alter the oriC-terC symmetry of the chromosome and the local genetic environment.

* Corresponding author. Mailing address: LMGM du CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex, France. Phone: (33) 561 33 58 25. Fax: (33) 561 33 58 86. E-mail: ritzenth{at}ibgc.biotoul.fr.

Journal of Bacteriology, September 1998, p. 4834-4842, Vol. 180, No. 18
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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