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Journal of Bacteriology, October 1998, p. 5010-5019, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A New Class of Caulobacter crescentus Flagellar Genes

Guy Leclerc,* Shui Ping Wang,dagger and Bert Ely

Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208

Received 27 March 1998/Accepted 24 July 1998

Eight Caulobacter crescentus flagellar genes, flmA, flmB, flmC, flmD, flmE, flmF, flmG, and flmH, have been cloned and characterized. These eight genes are clustered in pairs (flmAB, flmCD, flmEF, and flmGH) that appear to be structurally organized as operons. Homology comparisons suggest that the proteins encoded by the flm genes may be involved in posttranslational modification of flagellins or proteins that interact with flagellin monomers prior to their assembly into a flagellar filament. Expression of the flmAB, flmEF, and flmGH operons was shown to occur primarily in predivisional cells. In contrast, the flmCD operon was expressed throughout the cell cycle, with only a twofold increase in predivisional cells. The expression of the three temporally regulated operons was subject to positive regulation by the CtrA response regulator protein. Mutations in class II and III flagellar genes had no significant effect on the expression of the flm genes. Furthermore, the flm genes did not affect the expression of class II or class III flagellar genes. However, mutations in the flm genes did result in reduced synthesis of the class IV flagellin proteins. Taken together, these data indicate that the flm operons belong to a new class of flagellar genes.

* Corresponding author. Mailing address: Department of Biological Sciences, University of South Carolina, 700 Sumter St., Columbia, SC 29208. Phone: (803) 777-7105. Fax: (803) 777-4002. E-mail: gleclerc{at}biol.sc.edu.

dagger Present address: Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110.

Journal of Bacteriology, October 1998, p. 5010-5019, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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