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Journal of Bacteriology, October 1998, p. 5020-5029, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Isolation of Candida glabrata Homologs of the Saccharomyces cerevisiae KRE9 and KNH1 Genes and Their Involvement in Cell Wall beta -1,6-Glucan Synthesis

Shigehisa Nagahashi,dagger Marc Lussier, and Howard Bussey*

Department of Biology, McGill University, Montréal, Quebec, Canada H3A 1B1

Received 18 May 1998/Accepted 28 July 1998

The Candida glabrata KRE9 (CgKRE9) and KNH1 (CgKNH1) genes have been isolated as multicopy suppressors of the tetracycline-sensitive growth of a Saccharomyces cerevisiae mutant with the disrupted KNH1 locus and the KRE9 gene placed under the control of a tetracycline-responsive promoter. Although a cgknh1Delta mutant showed no phenotype beyond slightly increased sensitivity to the K1 killer toxin, disruption of CgKRE9 resulted in several phenotypes similar to those of the S. cerevisiae kre9Delta null mutant: a severe growth defect on glucose medium, resistance to the K1 killer toxin, a 50% reduction of beta -1,6-glucan, and the presence of aggregates of cells with abnormal morphology on glucose medium. Replacement in C. glabrata of the cognate CgKRE9 promoter with the tetracycline-responsive promoter in a cgknh1Delta background rendered cell growth tetracycline sensitive on media containing glucose or galactose. cgkre9Delta cells were shown to be sensitive to calcofluor white specifically on glucose medium. In cgkre9 mutants grown on glucose medium, cellular chitin levels were massively increased.

* Corresponding author. Mailing address: Department of Biology, McGill University, 1205 Dr. Penfield Ave., Montréal, Quebec, Canada H3A 1B1. Phone: (514) 398-6439. Fax: (514) 398-2595. E-mail: hbussey{at}monod.biol.mcgill.ca.

dagger Present address: Department of Mycology, Nippon Roche Research Center, Kamakura, Kanagawa 247-8530, Japan.

Journal of Bacteriology, October 1998, p. 5020-5029, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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