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Journal of Bacteriology, October 1998, p. 5030-5037, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

New Potential Cell Wall Glucanases of Saccharomyces cerevisiae and Their Involvement in Mating

Corinna Cappellaro,dagger Vladimir Mrsa, and Widmar Tanner*

Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, 93040 Regensburg, Germany

Received 28 May 1998/Accepted 3 August 1998

Biotinylation of intact Saccharomyces cerevisiae cells with a nonpermeant reagent (Sulfo-NHS-LC-Biotin) allowed the identification of seven cell wall proteins that were released from intact cells by dithiothreitol (DTT). By N-terminal sequencing, three of these proteins were identified as the known proteins beta -exoglucanase 1 (Exg1p), beta -endoglucanase (Bgl2p), and chitinase (Cts1p). One protein was related to the PIR protein family, whereas the remaining three (Scw3p, Scw4p, and Scw10p [for soluble cell wall proteins]) were found to be related to glucanases. Single knockouts of these three potential glucanases did not result in dramatic phenotypes. The double knockout of SCW4 and the homologous gene SCW10 resulted in slower growth, significantly increased release of proteins from intact cells by DTT, and highly decreased mating efficiency when these two genes were disrupted in both mating types. The synergistic behavior of the disruption of SCW4 and SCW10 was partly antagonized by the disruption of BGL2. The data are discussed in terms of a possible counterplay of transglucosidase and glucosidase activities.

* Corresponding author. Mailing address: Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, 93040 Regensburg, Germany. Phone: 49 941 943 3018. Fax: 49 941 943 3352. E-mail: widmar.tanner{at}biologie.uni-regensburg.de.

dagger Present address: Institut für molekulare Genetik, 37077 Göttingen, Germany.

Journal of Bacteriology, October 1998, p. 5030-5037, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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