This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tweeddale, H.
Right arrow Articles by Ferenci, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tweeddale, H.
Right arrow Articles by Ferenci, T.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 1998, p. 5109-5116, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Effect of Slow Growth on Metabolism of Escherichia coli, as Revealed by Global Metabolite Pool ("Metabolome") Analysis

Helen Tweeddale, Lucinda Notley-McRobb, and Thomas Ferenci*

Department of Microbiology, University of Sydney, New South Wales 2006, Australia

Received 6 May 1998/Accepted 28 July 1998

Escherichia coli growing on glucose in minimal medium controls its metabolite pools in response to environmental conditions. The extent of pool changes was followed through two-dimensional thin-layer chromatography of all 14C-glucose labelled compounds extracted from bacteria. The patterns of metabolites and spot intensities detected by phosphorimaging were found to reproducibly differ depending on culture conditions. Clear trends were apparent in the pool sizes of several of the 70 most abundant metabolites extracted from bacteria growing in glucose-limited chemostats at different growth rates. The pools of glutamate, aspartate, trehalose, and adenosine as well as UDP-sugars and putrescine changed markedly. The data on pools observed by two-dimensional thin-layer chromatography were confirmed for amino acids by independent analysis. Other unidentified metabolites also displayed different spot intensities under various conditions, with four trend patterns depending on growth rate. As RpoS controls a number of metabolic genes in response to nutrient limitation, an rpoS mutant was also analyzed for metabolite pools. The mutant had altered metabolite profiles, but only some of the changes at slow growth rates were ascribable to the known control of metabolic genes by RpoS. These results indicate that total metabolite pool ("metabolome") analysis offers a means of revealing novel aspects of cellular metabolism and global regulation.

* Corresponding author. Mailing address: Department of Microbiology G08, University of Sydney, NSW 2006, Australia. Phone: 61-2-9351-4277. Fax: 61-2-9351-4571. E-mail: t.ferenci{at}microbio.su.oz.au.

Journal of Bacteriology, October 1998, p. 5109-5116, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lee, S. J., Trostel, A., Le, P., Harinarayanan, R., FitzGerald, P. C., Adhya, S. (2009). Cellular stress created by intermediary metabolite imbalances. Proc. Natl. Acad. Sci. USA 106: 19515-19520 [Abstract] [Full Text]  
  • Tremaroli, V., Workentine, M. L., Weljie, A. M., Vogel, H. J., Ceri, H., Viti, C., Tatti, E., Zhang, P., Hynes, A. P., Turner, R. J., Zannoni, D. (2009). Metabolomic Investigation of the Bacterial Response to a Metal Challenge. Appl. Environ. Microbiol. 75: 719-728 [Abstract] [Full Text]  
  • Hasegawa, M., Ide, M., Fujita, T., Takenaka, S. (2008). Urinary Metabolic Fingerprinting for {alpha}-naphthylisothiocyanate-induced Intrahepatic Cholestasis in Rats Using Fourier Transform-ion Cyclotron Resonance Mass Spectrometry. Toxicol Pathol 36: 818-826 [Abstract] [Full Text]  
  • Fabich, A. J., Jones, S. A., Chowdhury, F. Z., Cernosek, A., Anderson, A., Smalley, D., McHargue, J. W., Hightower, G. A., Smith, J. T., Autieri, S. M., Leatham, M. P., Lins, J. J., Allen, R. L., Laux, D. C., Cohen, P. S., Conway, T. (2008). Comparison of Carbon Nutrition for Pathogenic and Commensal Escherichia coli Strains in the Mouse Intestine. Infect. Immun. 76: 1143-1152 [Abstract] [Full Text]  
  • Buckstein, M. H., He, J., Rubin, H. (2008). Characterization of Nucleotide Pools as a Function of Physiological State in Escherichia coli. J. Bacteriol. 190: 718-726 [Abstract] [Full Text]  
  • Zhen, Y., Krausz, K. W., Chen, C., Idle, J. R., Gonzalez, F. J. (2007). Metabolomic and Genetic Analysis of Biomarkers for Peroxisome Proliferator-Activated Receptor {alpha} Expression and Activation. Mol. Endocrinol. 21: 2136-2151 [Abstract] [Full Text]  
  • Maharjan, R. P., Seeto, S., Ferenci, T. (2007). Divergence and Redundancy of Transport and Metabolic Rate-Yield Strategies in a Single Escherichia coli Population. J. Bacteriol. 189: 2350-2358 [Abstract] [Full Text]  
  • Goodacre, R. (2007). Metabolomics of a Superorganism. J. Nutr. 137: 259S-266S [Abstract] [Full Text]  
  • Franchini, A. G., Egli, T. (2006). Global gene expression in Escherichia coli K-12 during short-term and long-term adaptation to glucose-limited continuous culture conditions. Microbiology 152: 2111-2127 [Abstract] [Full Text]  
  • Shulaev, V. (2006). Metabolomics technology and bioinformatics. Brief Bioinform 7: 128-139 [Abstract] [Full Text]  
  • Patel, C. N., Wortham, B. W., Lines, J. L., Fetherston, J. D., Perry, R. D., Oliveira, M. A. (2006). Polyamines are essential for the formation of plague biofilm.. J. Bacteriol. 188: 2355-2363 [Abstract] [Full Text]  
  • Pieterse, B., Leer, R. J., Schuren, F. H. J., van der Werf, M. J. (2005). Unravelling the multiple effects of lactic acid stress on Lactobacillus plantarum by transcription profiling. Microbiology 151: 3881-3894 [Abstract] [Full Text]  
  • Robertson, D. G. (2005). Metabonomics in Toxicology: A Review. Toxicol Sci 85: 809-822 [Abstract] [Full Text]  
  • Hua, Q., Yang, C., Oshima, T., Mori, H., Shimizu, K. (2004). Analysis of Gene Expression in Escherichia coli in Response to Changes of Growth-Limiting Nutrient in Chemostat Cultures. Appl. Environ. Microbiol. 70: 2354-2366 [Abstract] [Full Text]  
  • Schneider, B. L., Ruback, S., Kiupakis, A. K., Kasbarian, H., Pybus, C., Reitzer, L. (2002). The Escherichia coli gabDTPC Operon: Specific {gamma}-Aminobutyrate Catabolism and Nonspecific Induction. J. Bacteriol. 184: 6976-6986 [Abstract] [Full Text]  
  • Liu, X., Ferenci, T. (2001). An analysis of multifactorial influences on the transcriptional control of ompF and ompC porin expression under nutrient limitation. Microbiology 147: 2981-2989 [Abstract] [Full Text]  
  • Watkins, S. M, Hammock, B. D, Newman, J. W, German, J B. (2001). Individual metabolism should guide agriculture toward foods for improved health and nutrition. Am. J. Clin. Nutr. 74: 283-286 [Abstract] [Full Text]  
  • Greenbaum, D., Luscombe, N. M., Jansen, R., Qian, J., Gerstein, M. (2001). Interrelating Different Types of Genomic Data, from Proteome to Secretome: 'Oming in on Function. Genome Res 11: 1463-1468 [Abstract] [Full Text]  
  • Ostrowski, M., Cavicchioli, R., Blaauw, M., Gottschal, J. C. (2001). Specific Growth Rate Plays a Critical Role in Hydrogen Peroxide Resistance of the Marine Oligotrophic Ultramicrobacterium Sphingomonas alaskensis Strain RB2256. Appl. Environ. Microbiol. 67: 1292-1299 [Abstract] [Full Text]  
  • Liu, X., Ng, C., Ferenci, T. (2000). Global Adaptations Resulting from High Population Densities in Escherichia coli Cultures. J. Bacteriol. 182: 4158-4164 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»