This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Scharf, B. E.
Right arrow Articles by Berg, H. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Scharf, B. E.
Right arrow Articles by Berg, H. C.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 1998, p. 5123-5128, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

CheZ Has No Effect on Flagellar Motors Activated by CheY13DK106YW

Birgit E. Scharf, Karen A. Fahrner, and Howard C. Berg*

Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138

Received 4 June 1998/Accepted 6 August 1998

The behaviors of both cheZ-deleted and wild-type cells of Escherichia coli were found to be very sensitive to the level of expression of CheZ, a protein known to accelerate the dephosphorylation of the response regulator CheY-phosphate (CheY-P). However, cells induced to run and tumble by the unphosphorylated mutant protein CheY13DK106YW (CheY**) failed to respond to CheZ, even when CheZ was expressed at high levels. Therefore, CheZ neither affects the flagellar motors directly nor sequesters CheY**. In in vitro cross-linking studies, CheY** promoted trimerization of CheZ to the same extent as wild-type CheY but failed to induce the formation of complexes of higher molecular weight observed with CheY-P. Also, CheY** could be cross-linked to FliM, the motor receptor protein, nearly as well as CheY-P. Thus, to CheZ, CheY** looks like CheY, but to FliM, it looks like CheY-P.

* Corresponding author. Mailing address: Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138. Phone: (617) 495-0924. Fax: (617) 496-1114. E-mail: hberg{at}biosun.harvard.edu.

Journal of Bacteriology, October 1998, p. 5123-5128, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Silversmith, R. E., Levin, M. D., Schilling, E., Bourret, R. B. (2008). Kinetic Characterization of Catalysis by the Chemotaxis Phosphatase CheZ: MODULATION OF ACTIVITY BY THE PHOSPHORYLATED CheY SUBSTRATE. J. Biol. Chem. 283: 756-765 [Abstract] [Full Text]  
  • Li, M., Hazelbauer, G. L. (2004). Cellular Stoichiometry of the Components of the Chemotaxis Signaling Complex. J. Bacteriol. 186: 3687-3694 [Abstract] [Full Text]  
  • Sourjik, V., Berg, H. C. (2002). Binding of the Escherichia coli response regulator CheY to its target measured in vivo by fluorescence resonance energy transfer. Proc. Natl. Acad. Sci. USA 99: 12669-12674 [Abstract] [Full Text]  
  • Bao, Q., Tian, Y., Li, W., Xu, Z., Xuan, Z., Hu, S., Dong, W., Yang, J., Chen, Y., Xue, Y., Xu, Y., Lai, X., Huang, L., Dong, X., Ma, Y., Ling, L., Tan, H., Chen, R., Wang, J., Yu, J., Yang, H. (2002). A Complete Sequence of the T. tengcongensis Genome. Genome Res 12: 689-700 [Abstract] [Full Text]  
  • Bren, A., Eisenbach, M. (2000). How Signals Are Heard during Bacterial Chemotaxis: Protein-Protein Interactions in Sensory Signal Propagation. J. Bacteriol. 182: 6865-6873 [Full Text]  
  • Cluzel, P., Surette, M., Leibler, S. (2000). An Ultrasensitive Bacterial Motor Revealed by Monitoring Signaling Proteins in Single Cells. Science 287: 1652-1655 [Abstract] [Full Text]  
  • Schuster, M., Zhao, R., Bourret, R. B., Collins, E. J. (2000). Correlated Switch Binding and Signaling in Bacterial Chemotaxis. J. Biol. Chem. 275: 19752-19758 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»