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Journal of Bacteriology, October 1998, p. 5129-5134, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
hrcA, Encoding the Repressor of the groEL
Genes in Streptomyces albus G, Is Associated with a Second
dnaJ Gene
Cosette
Grandvalet,
Georges
Rapoport, and
Philippe
Mazodier*
Unité de Biochimie Microbienne,
Institut Pasteur, 75724 Paris Cedex 15, France
Received 9 March 1998/Accepted 3 August 1998
Expression of the principal chaperones of the heat shock stimulon
of Streptomyces albus G are under the negative control of different repressors. The dnaK operon is regulated by
hspR, the last gene of the operon
(dnaK-grpE-dnaJ-hspR). hsp18, encoding a
member of the small heat shock protein family, is regulated by
orfY, which is in the opposite orientation upstream of
hsp18. The groES-groEL1 operon and the
groEL2 gene are regulated differently. They present tandem
copies of the CIRCE element found in the 5' region of many heat shock
genes and shown to act in Bacillus subtilis as an operator
for a repressor encoded by hrcA (hrc stands for heat regulation at CIRCE). We report the identification in S. albus of a new heat shock operon containing hrcA and
dnaJ homologs. Disruption of hrcA increased the
transcription of the groES-groEL1 operon and of the
groEL2 gene. These features were lost when the mutant was
complemented in trans by an intact copy of
hrcA. Despite considerable accumulation of the GroE
chaperones in the hrcA mutant, there was no effect on
formation of the aerial mycelium and sporulation, indicating that
neither hrcA nor the level of groE gene
expression is directly involved in the regulation of
Streptomyces morphological differentiation.
*
Corresponding author. Mailing address: Unité de
Biochimie Microbienne, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: 33-1 45 68 88 42. Fax: 33-1 45 68 89 38. E-mail: mazodier{at}pasteur.fr.
Journal of Bacteriology, October 1998, p. 5129-5134, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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