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Journal of Bacteriology, October 1998, p. 5227-5230, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Ndd, the Bacteriophage T4 Protein That Disrupts the Escherichia coli Nucleoid, Has a DNA Binding Activity

Jean-Yves Bouet,dagger Henry M. Krisch, and Jean-Michel Louarn*

Laboratoire de Microbiologie et de Génétique Moléculaire du CNRS, Toulouse, France

Received 8 January 1998/Accepted 23 July 1998

Early in a bacteriophage T4 infection, the phage ndd gene causes the rapid destruction of the structure of the Escherichia coli nucleoid. Even at very low levels, the Ndd protein is extremely toxic to cells. In uninfected E. coli, overexpression of the cloned ndd gene induces disruption of the nucleoid that is indistinguishable from that observed after T4 infection. A preliminary characterization of this protein indicates that it has a double-stranded DNA binding activity with a preference for bacterial DNA rather than phage T4 DNA. The targets of Ndd action may be the chromosomal sequences that determine the structure of the nucleoid.

* Corresponding author. Mailing address: Laboratoire de Microbiologie et de Génétique Moléculaire du CNRS, 118, route de Narbonne, Toulouse 31062 Cédex, France. Phone: (33) 561-33-59-64. Fax: (33) 561-33-58-86. E-mail: louarn{at}ibcg.biotoul.fr.

dagger Present address: Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.

Journal of Bacteriology, October 1998, p. 5227-5230, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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