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Journal of Bacteriology, October 1998, p. 5247-5250, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Suppression of the ptsH Mutation in Escherichia coli and Salmonella typhimurium by a DNA Fragment from Lactobacillus casei

Vicente Monedero,1 Pieter W. Postma,2 and Gaspar Pérez-Martínez1,*

Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, 46100-Burjassot, Valencia, Spain,1 and E. C. Slater Instituut, BioCentrum, University of Amsterdam, 1018 TV Amsterdam, The Netherlands2

Received 12 June 1998/Accepted 24 July 1998

A DNA fragment from Lactobacillus casei that restores growth to Escherichia coli and Salmonella typhimurium ptsH mutants on glucose and other substrates of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) has been isolated. These mutants lack the HPr protein, a general component of the PTS. Sequencing of the cloned fragment revealed the absence of ptsH homologues. Instead, the complementation ability was located in a 120-bp fragment that contained a sequence homologue to the binding site of the Cra regulator from enteric bacteria. Experiments indicated that the reversion of the ptsH phenotype was due to a titration of the Cra protein, which allowed the constitutive expression of the fructose operon.

* Corresponding author. Mailing address: Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Polígono de la Coma s/n, Apartado de correos 73, 46100-Burjassot, Valencia, Spain. Phone: 34 6 3900022. Fax: 34 6 3636301. E-mail: gaspar.perez{at}iata.csic.es.

Journal of Bacteriology, October 1998, p. 5247-5250, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.




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