This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nellen-Anthamatten, D.
Right arrow Articles by Hennecke, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nellen-Anthamatten, D.
Right arrow Articles by Hennecke, H.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 1998, p. 5251-5255, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Bradyrhizobium japonicum FixK2, a Crucial Distributor in the FixLJ-Dependent Regulatory Cascade for Control of Genes Inducible by Low Oxygen Levels

D. Nellen-Anthamatten, P. Rossi, O. Preisig, I. Kullik, M. Babst, H. M. Fischer, and H. Hennecke*

Mikrobiologisches Institut, Eidgenössische Technische Hochschule, CH-8092 Zürich, Switzerland

Received 16 March 1998/Accepted 3 July 1998

Bradyrhizobium japonicum possesses a second fixK-like gene, fixK2, in addition to the previously identified fixK1 gene. The expression of both genes depends in a hierarchical fashion on the low-oxygen-responsive two-component regulatory system FixLJ, whereby FixJ first activates fixK2, whose product then activates fixK1. While the target genes for control by FixK1 are unknown, there is evidence for activation of the fixNOQP, fixGHIS, and rpoN1 genes and some heme biosynthesis and nitrate respiration genes by FixK2. FixK2 also regulates its own structural gene, directly or indirectly, in a negative way.


* Corresponding author. Mailing address: Mikrobiologisches Institut, Eidgenössische Technische Hochschule, ETH-Zentrum, Schmelzbergstrasse 7, CH-8092 Zürich, Switzerland. Phone: 41-1-632 3318. Fax: 41-1-632 1382. E-mail: hennecke{at}micro.biol.ethz.ch.


Journal of Bacteriology, October 1998, p. 5251-5255, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Mesa, S., Hauser, F., Friberg, M., Malaguti, E., Fischer, H.-M., Hennecke, H. (2008). Comprehensive Assessment of the Regulons Controlled by the FixLJ-FixK2-FixK1 Cascade in Bradyrhizobium japonicum. J. Bacteriol. 190: 6568-6579 [Abstract] [Full Text]  
  • Wei, M., Yokoyama, T., Minamisawa, K., Mitsui, H., Itakura, M., Kaneko, T., Tabata, S., Saeki, K., Omori, H., Tajima, S., Uchiumi, T., Abe, M., Ohwada, T. (2008). Soybean Seed Extracts Preferentially Express Genomic Loci of Bradyrhizobium japonicum in the Initial Interaction with Soybean, Glycine max (L.) Merr. DNA Res 15: 201-214 [Abstract] [Full Text]  
  • Lindemann, A., Moser, A., Pessi, G., Hauser, F., Friberg, M., Hennecke, H., Fischer, H.-M. (2007). New Target Genes Controlled by the Bradyrhizobium japonicum Two-Component Regulatory System RegSR. J. Bacteriol. 189: 8928-8943 [Abstract] [Full Text]  
  • Conlan, S., Lawrence, C., McCue, L. A. (2005). Rhodopseudomonas palustris Regulons Detected by Cross-Species Analysis of Alphaproteobacterial Genomes. Appl. Environ. Microbiol. 71: 7442-7452 [Abstract] [Full Text]  
  • Mesa, S., Ucurum, Z., Hennecke, H., Fischer, H.-M. (2005). Transcription Activation In Vitro by the Bradyrhizobium japonicum Regulatory Protein FixK2. J. Bacteriol. 187: 3329-3338 [Abstract] [Full Text]  
  • Balland, V., Bouzhir-Sima, L., Kiger, L., Marden, M. C., Vos, M. H., Liebl, U., Mattioli, T. A. (2005). Role of Arginine 220 in the Oxygen Sensor FixL from Bradyrhizobium japonicum. J. Biol. Chem. 280: 15279-15288 [Abstract] [Full Text]  
  • Ferrieres, L., Francez-Charlot, A., Gouzy, J., Rouille, S., Kahn, D. (2004). FixJ-regulated genes evolved through promoter duplication in Sinorhizobium meliloti. Microbiology 150: 2335-2345 [Abstract] [Full Text]  
  • Moris, M., Dombrecht, B., Xi, C., Vanderleyden, J., Michiels, J. (2004). Regulatory Role of Rhizobium etli CNPAF512 fnrN during Symbiosis. Appl. Environ. Microbiol. 70: 1287-1296 [Abstract] [Full Text]  
  • Delgado, M. J., Bonnard, N., Tresierra-Ayala, A., Bedmar, E. J., Muller, P. (2003). The Bradyrhizobium japonicum napEDABC genes encoding the periplasmic nitrate reductase are essential for nitrate respiration. Microbiology 149: 3395-3403 [Abstract] [Full Text]  
  • Sciotti, M.-A., Chanfon, A., Hennecke, H., Fischer, H.-M. (2003). Disparate Oxygen Responsiveness of Two Regulatory Cascades That Control Expression of Symbiotic Genes in Bradyrhizobium japonicum. J. Bacteriol. 185: 5639-5642 [Abstract] [Full Text]  
  • Mesa, S., Bedmar, E. J., Chanfon, A., Hennecke, H., Fischer, H.-M. (2003). Bradyrhizobium japonicum NnrR, a Denitrification Regulator, Expands the FixLJ-FixK2 Regulatory Cascade. J. Bacteriol. 185: 3978-3982 [Abstract] [Full Text]  
  • Jones, K. M., Buikema, W. J., Haselkorn, R. (2003). Heterocyst-Specific Expression of patB, a Gene Required for Nitrogen Fixation in Anabaena sp. Strain PCC 7120. J. Bacteriol. 185: 2306-2314 [Abstract] [Full Text]  
  • Mesa, S., Velasco, L., Manzanera, M. E., Delgado, M. J., Bedmar, E. J. (2002). Characterization of the norCBQD genes, encoding nitric oxide reductase, in the nitrogen fixing bacterium Bradyrhizobium japonicum. Microbiology 148: 3553-3560 [Abstract] [Full Text]  
  • Arnesano, F., Banci, L., Bertini, I., Ciofi-Baffoni, S., Molteni, E., Huffman, D. L., O'Halloran, T. V. (2002). Metallochaperones and Metal-Transporting ATPases: A Comparative Analysis of Sequences and Structures. Genome Res 12: 255-271 [Abstract] [Full Text]  
  • Lopez, O., Morera, C., Miranda-Rios, J., Girard, L., Romero, D., Soberon, M. (2001). Regulation of Gene Expression in Response to Oxygen in Rhizobium etli: Role of FnrN in fixNOQP Expression and in Symbiotic Nitrogen Fixation. J. Bacteriol. 183: 6999-7006 [Abstract] [Full Text]  
  • Yasuta, T., Okazaki, S., Mitsui, H., Yuhashi, K.-I., Ezura, H., Minamisawa, K. (2001). DNA Sequence and Mutational Analysis of Rhizobitoxine Biosynthesis Genes in Bradyrhizobium elkanii. Appl. Environ. Microbiol. 67: 4999-5009 [Abstract] [Full Text]  
  • Fischer, H.-M., Velasco, L., Delgado, M. J., Bedmar, E. J., Schären, S., Zingg, D., Göttfert, M., Hennecke, H. (2001). One of Two hemN Genes in Bradyrhizobium japonicum Is Functional during Anaerobic Growth and in Symbiosis. J. Bacteriol. 183: 1300-1311 [Abstract] [Full Text]  
  • Nienaber, A., Huber, A., Göttfert, M., Hennecke, H., Fischer, H.-M. (2000). Three New NifA-Regulated Genes in the Bradyrhizobium japonicum Symbiotic Gene Region Discovered by Competitive DNA-RNA Hybridization. J. Bacteriol. 182: 1472-1480 [Abstract] [Full Text]