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Journal of Bacteriology, October 1998, p. 5256-5259, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of Multiple sigma 54-Dependent Transcriptional Activators in Vibrio cholerae

Karl E. Klose,1,* Veronica Novik,2 and John J. Mekalanos2,3

Department of Microbiology, University of Texas Health Science Center, San Antonio, Texas 78284-7758,1 and Department of Microbiology and Molecular Genetics2 and Shipley Institute of Medicine,3 Harvard Medical School, Boston, Massachusetts 02115

Received 12 June 1998/Accepted 29 July 1998

In the pathogenic bacterium Vibrio cholerae, the alternate sigma factor sigma 54 is required for expression of multiple sets of genes, including an unidentified gene(s) necessary for enhanced colonization within the host. To identify sigma 54-dependent transcriptional activators involved in colonization, PCR was performed with V. cholerae chromosomal DNA and degenerate primers, revealing six novel and distinct coding sequences with homology to sigma 54-dependent activators. One sequence had high homology to the luxO gene of V. harveyi, which in that organism is involved in quorum sensing. Phenotypes of V. cholerae strains containing mutations in each of the six putative sigma 54-dependent activator genes identified one as a probable ntrC homologue. None of the mutant strains exhibited a defect in the ability to colonize infant mice, suggesting the presence of additional sigma 54-dependent activators not identified by this technique.

* Corresponding author. Mailing address: Department of Microbiology, University of Texas Health Science Center, 7703 Floyd Curl Dr., San Antonio, TX 78284-7758. Phone: (210) 567-3990. Fax: (210) 567-6612. E-mail: klose{at}uthscsa.edu.

Journal of Bacteriology, October 1998, p. 5256-5259, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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