This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Srinivasan, S. Articles by Kjelleberg, S. Search for Related Content PubMed PubMed Citation Articles by Srinivasan, S. Articles by Kjelleberg, S.

 Previous Article  |  Next Article 

J. Bacteriol., 01 1998, 201-209, Vol 180, No. 2
Copyright © 1998, American Society for Microbiology

Extracellular signal molecule(s) involved in the carbon starvation response of marine Vibrio sp. strain S14 [In Process Citation]

S Srinivasan, J Ostling, T Charlton, R de Nys, K Takayama and S Kjelleberg
School of Microbiology and Immunology, University of New South Wales, Sydney, Australia.

The role of exogenous metabolites as putative signal molecules mediating and/or regulating the carbon starvation adaptation program in Vibrio sp. strain S14 was investigated. Addition of the stationary- phase supernatant extract (SSE) of Vibrio sp. strain S14 to logarithmic- phase cells resulted in a significant number of carbon starvation- induced proteins being up-regulated. Halogenated furanones, putative antagonists of acylated homoserine lactones (AHLs), inhibited the synthesis of proteins specifically induced upon carbon starvation. The effect of the furanone was the opposite of that caused by SSE with respect to the up- and down-regulation of protein expression, indicating that both the furanone and the putative signalling molecules were acting on the same regulatory pathway. Culturability was rapidly lost when Vibrio sp. strain S14 was starved in the presence of the furanone at a low concentration. The furanone also had a negative effect on the ability of carbon-starved cells to mount resistance against UV irradiation and hydrogen peroxide exposure. The SSE of Vibrio sp. strain S14 had the ability to provide cross-protection against the loss in viability caused by the furanone. We have further demonstrated that the SSE taken from low- as well as high-cell-density cultures of Vibrio sp. strain S14 induced luminescence in Vibrio harveyi. Taken together, the results in this report provide evidence that Vibrio sp. strain S14 produces extracellular signalling metabolites during carbon and energy starvation and that these molecules play an important role in the expression of proteins crucial to the development of starvation- and stress-resistant phenotypes.

This article has been cited by other articles:

• Erickson, D. L., Lines, J. L., Pesci, E. C., Venturi, V., Storey, D. G. (2004). Pseudomonas aeruginosa relA Contributes to Virulence in Drosophila melanogaster. Infect. Immun. 72: 5638-5645 [Abstract] [Full Text]  
• Taylor, M. W., Schupp, P. J., Baillie, H. J., Charlton, T. S., de Nys, R., Kjelleberg, S., Steinberg, P. D. (2004). Evidence for Acyl Homoserine Lactone Signal Production in Bacteria Associated with Marine Sponges. Appl. Environ. Microbiol. 70: 4387-4389 [Abstract] [Full Text]  
• King, G. M. (2003). Uptake of Carbon Monoxide and Hydrogen at Environmentally Relevant Concentrations by Mycobacteria{dagger}. Appl. Environ. Microbiol. 69: 7266-7272 [Abstract] [Full Text]  
• McDougald, D., Srinivasan, S., Rice, S. A., Kjelleberg, S. (2003). Signal-mediated cross-talk regulates stress adaptation in Vibrio species. Microbiology 149: 1923-1933 [Abstract] [Full Text]  
• Weichart, D. H., Kell, D. B. (2001). Characterization of an autostimulatory substance produced by Escherichia coli. Microbiology 147: 1875-1885 [Abstract] [Full Text]  
• Bollinger, N., Hassett, D. J., Iglewski, B. H., Costerton, J. W., McDermott, T. R. (2001). Gene Expression in Pseudomonas aeruginosa: Evidence of Iron Override Effects on Quorum Sensing and Biofilm-Specific Gene Regulation. J. Bacteriol. 183: 1990-1996 [Abstract] [Full Text]  
• McDougald, D., Rice, S. A., Kjelleberg, S. (2001). SmcR-Dependent Regulation of Adaptive Phenotypes in Vibrio vulnificus. J. Bacteriol. 183: 758-762 [Abstract] [Full Text]  
• Hild, E., Takayama, K., Olsson, R.-M., Kjelleberg, S. (2000). Evidence for a Role of rpoE in Stressed and Unstressed Cells of Marine Vibrio angustum Strain S14. J. Bacteriol. 182: 6964-6974 [Abstract] [Full Text]  
• Thorne, S. H., Williams, H. D. (1999). Cell Density-Dependent Starvation Survival of Rhizobium leguminosarum bv. phaseoli: Identification of the Role of an N-Acyl Homoserine Lactone in Adaptation to Stationary-Phase Survival. J. Bacteriol. 181: 981-990 [Abstract] [Full Text]