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Journal of Bacteriology, October 1998, p. 5463-5465, Vol. 180, No. 20
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Autoregulation of the pTF-FC2 Proteic Poison-Antidote Plasmid Addiction System (pas) Is Essential for Plasmid Stabilization

Anthony S. G. Smith and Douglas E. Rawlings*

Department of Microbiology, University of Cape Town, Rondebosch 7700, South Africa

Received 13 February 1998/Accepted 22 June 1998

The pasABC genes of the proteic plasmid addiction system of broad-host-range plasmid pTF-FC2 were autoregulated. The PasA antidote was able to repress the operon 25-fold on its own, and repression was increased to 100-fold when the PasB toxin was also present. Autoregulation appears to be an essential requirement for pas-mediated plasmid stabilization because when the pas genes were placed behind the isopropyl-beta -D-thiogalactopyranoside (IPTG)-regulated tac promoter, they were unable to stabilize a heterologous test plasmid.

* Corresponding author. Present address: Department of Microbiology, University of Stellenbosch, Stellenbosch 7600, South Africa. Phone: 27 21 808 4866. Fax: 27 21 808 3611. E-mail: der{at}maties.sun.ac.za.

Journal of Bacteriology, October 1998, p. 5463-5465, Vol. 180, No. 20
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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