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Journal of Bacteriology, October 1998, p. 5473-5477, Vol. 180, No. 20
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of the mIHF Gene of Mycobacterium smegmatis

Marisa L. Pedulladagger and Graham F. Hatfull*

Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260

Received 6 May 1998/Accepted 5 August 1998

Integration of mycobacteriophage L5 requires the mycobacterial integration host factor (mIHF) in vitro. mIHF is a 105-residue heat-stable polypeptide that is not obviously related to HU or any other small DNA-binding proteins. mIHF is most abundant just prior to entry into stationary phase and is essential for the viability of Mycobacterium smegmatis.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260. Phone: (412) 624-6975. Fax: (412) 624-4870. E-mail: gfh{at}vms.cis.pitt.edu.

dagger Present address: Department of Biochemistry and Biophysics, Washington State University, Pullman, WA 99164.

Journal of Bacteriology, October 1998, p. 5473-5477, Vol. 180, No. 20
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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