Journal of Bacteriology, October 1998, p. 5478-5483, Vol. 180, No. 20
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Canadian Bacterial Disease Network, Department of Microbiology, College of Biological Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Received 10 June 1998/Accepted 11 August 1998
Naturally produced membrane vesicles (MVs), isolated from 15 strains of gram-negative bacteria (Citrobacter,
Enterobacter, Escherichia,
Klebsiella, Morganella, Proteus,
Salmonella, and Shigella strains), lysed many
gram-positive (including Mycobacterium) and
gram-negative cultures. Peptidoglycan zymograms suggested that MVs
contained peptidoglycan hydrolases, and electron microscopy revealed
that the murein sacculi were digested, confirming a previous modus
operandi (J. L. Kadurugamuwa and T. J. Beveridge, J. Bacteriol. 174:2767-2774, 1996). MV-sensitive bacteria possessed
A1
, A4
, A1
, A2
, and A4
peptidoglycan chemotypes, whereas
A3
, A3
, A3
, A4
, B1
, and B1
chemotypes were not
affected. Pseudomonas aeruginosa PAO1 vesicles possessed
the most lytic activity.
Present address: Department of Microbiology and Immunology, Temple
University School of Medicine, Philadelphia, PA 19140.
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