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Journal of Bacteriology, October 1998, p. 5478-5483, Vol. 180, No. 20
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Gram-Negative Bacteria Produce Membrane Vesicles Which Are Capable of Killing Other Bacteria

Zusheng Li,dagger Anthony J. Clarke, and Terry J. Beveridge*

Canadian Bacterial Disease Network, Department of Microbiology, College of Biological Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1

Received 10 June 1998/Accepted 11 August 1998

Naturally produced membrane vesicles (MVs), isolated from 15 strains of gram-negative bacteria (Citrobacter, Enterobacter, Escherichia, Klebsiella, Morganella, Proteus, Salmonella, and Shigella strains), lysed many gram-positive (including Mycobacterium) and gram-negative cultures. Peptidoglycan zymograms suggested that MVs contained peptidoglycan hydrolases, and electron microscopy revealed that the murein sacculi were digested, confirming a previous modus operandi (J. L. Kadurugamuwa and T. J. Beveridge, J. Bacteriol. 174:2767-2774, 1996). MV-sensitive bacteria possessed A1alpha , A4alpha , A1gamma , A2alpha , and A4gamma peptidoglycan chemotypes, whereas A3alpha , A3beta , A3gamma , A4beta , B1alpha , and B1beta chemotypes were not affected. Pseudomonas aeruginosa PAO1 vesicles possessed the most lytic activity.

* Corresponding author. Mailing address: Department of Microbiology, College of Biological Science, University of Guelph, Guelph, Ont. N1G 2W1, Canada. Phone: (519) 824-4120, ext. 3366. Fax: (519) 837-1802. E-mail: tjb{at}micro.uoguelph.ca.

dagger Present address: Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140.

Journal of Bacteriology, October 1998, p. 5478-5483, Vol. 180, No. 20
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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