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Journal of Bacteriology, November 1998, p. 5749-5755, Vol. 180, No. 21
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Subcellular Localization of Bacillus subtilis SMC, a Protein Involved in Chromosome Condensation and Segregation

Peter L. Graumann,1,* Richard Losick,1 and Alexander V. Strunnikov2

Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138,1 and National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 208922

Received 5 June 1998/Accepted 10 August 1998

We have investigated the subcellular localization of the SMC protein in the gram-positive bacterium Bacillus subtilis. Recent work has shown that SMC is required for chromosome condensation and faithful chromosome segregation during the B. subtilis cell cycle. Using antibodies against SMC and fluorescence microscopy, we have shown that SMC is associated with the chromosome but is also present in discrete foci near the poles of the cell. DNase treatment of permeabilized cells disrupted the association of SMC with the chromosome but not with the polar foci. The use of a truncated smc gene demonstrated that the C-terminal domain of the protein is required for chromosomal binding but not for the formation of polar foci. Regular arrays of SMC-containing foci were still present between nucleoids along the length of aseptate filaments generated by depleting cells of the cell division protein FtsZ, indicating that the formation of polar foci does not require the formation of septal structures. In slowly growing cells, which have only one or two chromosomes, SMC foci were principally observed early in the cell cycle, prior to or coincident with chromosome segregation. Cell cycle-dependent release of stored SMC from polar foci may mediate segregation by condensation of chromosomes.

* Corresponding author. Mailing address: Biological Laboratories, 16 Divinity Ave., Harvard University, Cambridge, MA 02138. Phone: (617) 495-0532. Fax: (617) 495-9300. E-mail: graumann{at}fas.harvard.edu.

Journal of Bacteriology, November 1998, p. 5749-5755, Vol. 180, No. 21
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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