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Journal of Bacteriology, November 1998, p. 5762-5764, Vol. 180, No. 21
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Deletion of the 389 N-Terminal Residues of the Transcriptional Activator AREA Does Not Result in Nitrogen Metabolite Derepression in Aspergillus nidulans

Mark X. Caddick1 and Herbert N. Arst Jr.2,*

School of Biological Sciences, Donnan Laboratories, University of Liverpool, Liverpool L69 7ZD,1 and Department of Infectious Diseases, Imperial College School of Medicine at Hammersmith Hospital, London W12 0NN,2 United Kingdom

Received 29 May 1998/Accepted 23 July 1998

Utilizing a homologous gene replacement in order to retain the native promoter and 5' and 3' untranslated messenger regions (and thereby ensure physiological validity), we have shown that deletion of the N-terminal 389 amino acids of the transcriptional activator AREA does not result in nitrogen metabolite derepression in Aspergillus nidulans. Our results provide no evidence for a modulating interaction involving the N terminus of AREA and contrast with those of H. K. Lamb, A. L. Dodds, D. R. Swatman, E. Cairns, and A. R. Hawkins (J. Bacteriol. 179:6649-6656, 1997), who used nontargeted ectopic copies of a construct containing a heterologous promoter and untranslated regions. Results obtained with this deletion mutant, nevertheless, provide further evidence for the dispensability of large portions of AREA.

* Corresponding author. Mailing address: Department of Infectious Diseases, Imperial College School of Medicine at Hammersmith Hospital, Ducane Rd., London W12 0NN, United Kingdom. Phone: (44) (181) 383 3436. Fax: (44) (181) 383 3394.

Journal of Bacteriology, November 1998, p. 5762-5764, Vol. 180, No. 21
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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