This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, Y.-Y. M.
Right arrow Articles by Burne, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, Y.-Y. M.
Right arrow Articles by Burne, R. A.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 1998, p. 5769-5775, Vol. 180, No. 21
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Transcriptional Regulation of the Streptococcus salivarius 57.I Urease Operon

Yi-Ywan M. Chen,1,2 Cheryl A. Weaver,1 David R. Mendelsohn,1 and Robert A. Burne1,2,*

Center for Oral Biology1 and Department of Microbiology and Immunology,2 School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642

Received 29 May 1998/Accepted 26 August 1998

The Streptococcus salivarius 57.I ure cluster was organized as an operon, beginning with ureI, followed by ureABC (structural genes) and ureEFGD (accessory genes). Northern analyses revealed transcripts encompassing structural genes and transcripts containing the entire operon. A sigma 70-like promoter could be mapped 5' to ureI (PureI) by primer extension analysis. The intensity of the signal increased when cells were grown at an acidic pH and was further enhanced by excess carbohydrate. To determine the function(s) of two inverted repeats located 5' to PureI, transcriptional fusions of the full-length promoter region (PureI), or a deletion derivative (PureIDelta 100), and a promoterless chloramphenicol acetyltransferase (CAT) gene were constructed and integrated into the chromosome to generate strains PureICAT and PureIDelta 100CAT, respectively. CAT specific activities of PureICAT were repressed at pH 7.0 and induced at pH 5.5 and by excess carbohydrate. In PureIDelta 100CAT, CAT activity was 60-fold higher than in PureICAT at pH 7.0 and pH induction was nearly eliminated, indicating that expression was negatively regulated. Thus, it was concluded that PureI was the predominant, regulated promoter and that regulation was governed by a mechanism differing markedly from other known mechanisms for bacterial urease expression.

* Corresponding author. Mailing address: Center for Oral Biology, University of Rochester, School of Medicine and Dentistry, 601 Elmwood Ave., Rochester, NY 14642. Phone: (716) 275-0381. Fax: (716) 473-2679. E-mail: robert_burne{at}urmc.rochester.edu.

Journal of Bacteriology, November 1998, p. 5769-5775, Vol. 180, No. 21
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Liu, Y., Zeng, L., Burne, R. A. (2009). AguR Is Required for Induction of the Streptococcus mutans Agmatine Deiminase System by Low pH and Agmatine. Appl. Environ. Microbiol. 75: 2629-2637 [Abstract] [Full Text]  
  • Liu, Y., Dong, Y., Chen, Y.-Y. M., Burne, R. A. (2008). Environmental and Growth Phase Regulation of the Streptococcus gordonii Arginine Deiminase Genes. Appl. Environ. Microbiol. 74: 5023-5030 [Abstract] [Full Text]  
  • Arioli, S., Monnet, C., Guglielmetti, S., Parini, C., De Noni, I., Hogenboom, J., Halami, P. M., Mora, D. (2007). Aspartate Biosynthesis Is Essential for the Growth of Streptococcus thermophilus in Milk, and Aspartate Availability Modulates the Level of Urease Activity. Appl. Environ. Microbiol. 73: 5789-5796 [Abstract] [Full Text]  
  • Ahn, S.-J., Burne, R. A. (2007). Effects of Oxygen on Biofilm Formation and the AtlA Autolysin of Streptococcus mutans. J. Bacteriol. 189: 6293-6302 [Abstract] [Full Text]  
  • Bore, E., Langsrud, S., Langsrud, O., Rode, T. M., Holck, A. (2007). Acid-shock responses in Staphylococcus aureus investigated by global gene expression analysis. Microbiology 153: 2289-2303 [Abstract] [Full Text]  
  • Lemos, J. A., Luzardo, Y., Burne, R. A. (2007). Physiologic Effects of Forced Down-Regulation of dnaK and groEL Expression in Streptococcus mutans. J. Bacteriol. 189: 1582-1588 [Abstract] [Full Text]  
  • Belzer, C., van Schendel, B. A. M., Kuipers, E. J., Kusters, J. G., van Vliet, A. H. M. (2007). Iron-Responsive Repression of Urease Expression in Helicobacter hepaticus Is Mediated by the Transcriptional Regulator Fur. Infect. Immun. 75: 745-752 [Abstract] [Full Text]  
  • Marri, P. R., Hao, W., Golding, G. B. (2006). Gene Gain and Gene Loss in Streptococcus: Is It Driven by Habitat?. Mol Biol Evol 23: 2379-2391 [Abstract] [Full Text]  
  • Ahn, S.-J., Burne, R. A. (2006). The atlA Operon of Streptococcus mutans: Role in Autolysin Maturation and Cell Surface Biogenesis.. J. Bacteriol. 188: 6877-6888 [Abstract] [Full Text]  
  • Ahn, S.-J., Lemos, J. A. C., Burne, R. A. (2005). Role of HtrA in Growth and Competence of Streptococcus mutans UA159. J. Bacteriol. 187: 3028-3038 [Abstract] [Full Text]  
  • Wen, Z. T., Suntharaligham, P., Cvitkovitch, D. G., Burne, R. A. (2005). Trigger Factor in Streptococcus mutans Is Involved in Stress Tolerance, Competence Development, and Biofilm Formation. Infect. Immun. 73: 219-225 [Abstract] [Full Text]  
  • Nascimento, M. M., Lemos, J. A. C., Abranches, J., Goncalves, R. B., Burne, R. A. (2004). Adaptive Acid Tolerance Response of Streptococcus sobrinus. J. Bacteriol. 186: 6383-6390 [Abstract] [Full Text]  
  • Wen, Z. T., Burne, R. A. (2004). LuxS-Mediated Signaling in Streptococcus mutans Is Involved in Regulation of Acid and Oxidative Stress Tolerance and Biofilm Formation. J. Bacteriol. 186: 2682-2691 [Abstract] [Full Text]  
  • Weeks, D. L., Gushansky, G., Scott, D. R., Sachs, G. (2004). Mechanism of Proton Gating of a Urea Channel. J. Biol. Chem. 279: 9944-9950 [Abstract] [Full Text]  
  • Lemos, J. A. C., Brown, T. A. Jr., Burne, R. A. (2004). Effects of RelA on Key Virulence Properties of Planktonic and Biofilm Populations of Streptococcus mutans. Infect. Immun. 72: 1431-1440 [Abstract] [Full Text]  
  • Chen, Y.-Y. M., Burne, R. A. (2003). Identification and Characterization of the Nickel Uptake System for Urease Biogenesis in Streptococcus salivarius 57.I. J. Bacteriol. 185: 6773-6779 [Abstract] [Full Text]  
  • Cotter, P. D., Hill, C. (2003). Surviving the Acid Test: Responses of Gram-Positive Bacteria to Low pH. Microbiol. Mol. Biol. Rev. 67: 429-453 [Abstract] [Full Text]  
  • Abranches, J., Chen, Y.-Y. M., Burne, R. A. (2003). Characterization of Streptococcus mutans Strains Deficient in EIIABMan of the Sugar Phosphotransferase System. Appl. Environ. Microbiol. 69: 4760-4769 [Abstract] [Full Text]  
  • Chen, Y.-Y. M., Betzenhauser, M. J., Burne, R. A. (2002). cis-Acting elements that regulate the low-pH-inducible urease operon of Streptococcus salivarius. Microbiology 148: 3599-3608 [Abstract] [Full Text]  
  • Lemos, J. A. C., Chen, Y.-Y. M., Burne, R. A. (2001). Genetic and Physiologic Analysis of the groE Operon and Role of the HrcA Repressor in Stress Gene Regulation and Acid Tolerance in Streptococcus mutans. J. Bacteriol. 183: 6074-6084 [Abstract] [Full Text]  
  • Li, Y., Burne, R. A. (2001). Regulation of the gtfBC and ftf genes of Streptococcus mutans in biofilms in response to pH and carbohydrate. Microbiology 147: 2841-2848 [Abstract] [Full Text]  
  • van Vliet, A. H. M., Kuipers, E. J., Waidner, B., Davies, B. J., de Vries, N., Penn, C. W., Vandenbroucke-Grauls, C. M. J. E., Kist, M., Bereswill, S., Kusters, J. G. (2001). Nickel-Responsive Induction of Urease Expression in Helicobacter pylori Is Mediated at the Transcriptional Level. Infect. Immun. 69: 4891-4897 [Abstract] [Full Text]  
  • Upton, M., Tagg, J. R., Wescombe, P., Jenkinson, H. F. (2001). Intra- and Interspecies Signaling between Streptococcus salivarius and Streptococcus pyogenes Mediated by SalA and SalA1 Lantibiotic Peptides. J. Bacteriol. 183: 3931-3938 [Abstract] [Full Text]  
  • Quivey, R. G. Jr, Kuhnert, W. L., Hahn, K. (2001). Genetics of Acid Adaptation in Oral Streptococci. CROBM 12: 301-314 [Abstract] [Full Text]  
  • Morou-Bermudez, E., Burne, R. A. (2000). Analysis of Urease Expression in Actinomyces naeslundii WVU45. Infect. Immun. 68: 6670-6676 [Abstract] [Full Text]  
  • Chen, Y.-Y. M., Weaver, C. A., Burne, R. A. (2000). Dual Functions of Streptococcus salivarius Urease. J. Bacteriol. 182: 4667-4669 [Abstract] [Full Text]  
  • Bergeron, L. J., Morou-Bermudez, E., Burne, R. A. (2000). Characterization of the Fructosyltransferase Gene of Actinomyces naeslundii WVU45. J. Bacteriol. 182: 3649-3654 [Abstract] [Full Text]  
  • Clancy, K. A., Pearson, S., Bowen, W. H., Burne, R. A. (2000). Characterization of Recombinant, Ureolytic Streptococcus mutans Demonstrates an Inverse Relationship between Dental Plaque Ureolytic Capacity and Cariogenicity. Infect. Immun. 68: 2621-2629 [Abstract] [Full Text]  
  • Weaver, C. A., Chen, Y.-Y. M., Burne, R. A. (2000). Inactivation of the ptsI gene encoding enzyme I of the sugar phosphotransferase system of Streptococcus salivarius: effects on growth and urease expression. Microbiology 146: 1179-1185 [Abstract] [Full Text]  
  • Burne, R. A., Wen, Z. T., Chen, Y.-Y. M., Penders, J. E. C. (1999). Regulation of Expression of the Fructan Hydrolase Gene of Streptococcus mutans GS-5 by Induction and Carbon Catabolite Repression. J. Bacteriol. 181: 2863-2871 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»