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Journal of Bacteriology, November 1998, p. 5788-5791, Vol. 180, No. 21
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Induction of ermAMR from a Clinical Strain of Enterococcus faecalis by 16-Membered-Ring Macrolide Antibiotics

Tae-Gwon Oh, Ae-Ran Kwon, and Eung-Chil Choi*

College of Pharmacy, Seoul National University, Seoul 151-742, Korea

Received 2 July 1998/Accepted 31 August 1998

We cloned the MLSB resistance determinant by PCR from a clinical isolate of Enterococcus faecalis 373, which is induced more strongly by a 16-membered-ring macrolide, tylosin, than by erythromycin. To elucidate the molecular basis of resistance of E. faecalis 373, we analyzed the cloned gene, designated ermAMR, by site-directed mutagenesis and reporter gene assay. Our results showed that an arginine-to-cysteine change in the seventh codon of the putative leader peptide endowed tylosin with resistance inducibility and that TAAA duplication enabled the control region to express the downstream methylase gene at a drastically increased level.

* Corresponding author. Mailing address: College of Pharmacy, Seoul National University, Seoul 151-742, Korea. Phone: 82-2-880-7874. Fax: 82-2-886-5802. E-mail: ecchoi{at}plaza.snu.ac.kr.

Journal of Bacteriology, November 1998, p. 5788-5791, Vol. 180, No. 21
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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