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Journal of Bacteriology, November 1998, p. 5796-5798, Vol. 180, No. 21
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Nucleotide Excision Repair in the Third Kingdom

Müge Ögrünç,1 Donald F. Becker,2 Stephen W. Ragsdale,2 and Aziz Sancar1,*

Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599,1 and Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 685882

Received 10 July 1998/Accepted 24 August 1998

Nucleotide excision repair, a general repair mechanism for removing DNA damage, is initiated by dual incisions bracketing the lesion. In procaryotes, the dual incisions result in excision of the damage in 12- to 13-nucleotide-long oligomers, and in eucaryotes they result in excision of the damage in the form of 24- to 32-nucleotide-long oligomers. We wished to find out if Archaea perform excision repair. Using cell extracts from Methanobacterium thermoautotrophicum, we found that this organism removes UV-induced (6-4) photoproducts in the form of 10- to 11-mers by incising the sixth to seventh phosphodiester bond 5' to the damage and the fourth phosphodiester bond 3' to the damage.

* Corresponding author. Mailing address: Department of Biochemistry and Biophysics, Mary Ellen Jones Building, CB# 7260, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7260. Phone: (919) 962-0115. Fax: (919) 966-2852.

Journal of Bacteriology, November 1998, p. 5796-5798, Vol. 180, No. 21
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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