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Journal of Bacteriology, November 1998, p. 5796-5798, Vol. 180, No. 21
Department of Biochemistry and Biophysics,
University of North Carolina School of Medicine, Chapel Hill, North
Carolina 27599,1 and
Department of
Biochemistry, University of Nebraska, Lincoln, Nebraska
685882
Received 10 July 1998/Accepted 24 August 1998
Nucleotide excision repair, a general repair mechanism for removing
DNA damage, is initiated by dual incisions bracketing the lesion.
In procaryotes, the dual incisions result in excision of the damage in
12- to 13-nucleotide-long oligomers, and in eucaryotes they result
in excision of the damage in the form of 24- to
32-nucleotide-long oligomers. We wished to find out if
Archaea perform excision repair. Using cell extracts from
Methanobacterium thermoautotrophicum, we found that this
organism removes UV-induced (6-4) photoproducts in the form of 10- to
11-mers by incising the sixth to seventh phosphodiester bond 5' to the
damage and the fourth phosphodiester bond 3' to the damage.
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Nucleotide Excision Repair in the Third
Kingdom
*
Corresponding author. Mailing address: Department of
Biochemistry and Biophysics, Mary Ellen Jones Building, CB# 7260, University of North Carolina at Chapel Hill, Chapel Hill, NC
27599-7260. Phone: (919) 962-0115. Fax: (919) 966-2852.
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