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Journal of Bacteriology, November 1998, p. 5855-5859, Vol. 180, No. 22
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

atp Mutants of Escherichia coli Fail To Grow on Succinate Due to a Transport Deficiency

Fred C. Boogerd,¹ Lars Boe,² Ole Michelsen,² and Peter Ruhdal Jensen^2,*

Department of Molecular Cell Physiology, Faculty of Biology, Free University, Amsterdam, The Netherlands,¹ and Department of Microbiology, Technical University of Denmark, Lyngby, Denmark²

Received 1 June 1998/Accepted 5 September 1998

Escherichia coli atp mutants, which lack a functional H⁺-ATPase complex, are capable of growth on glucose but not on succinate or other C₄-dicarboxylates (Suc phenotype). Suc⁺ revertants of an atp deletion strain were isolated which were capable of growth on succinate even though they lack the entire H⁺-ATPase complex. Complementation in trans with the yhiF gene suppressed the growth of the Suc⁺ mutants on succinate, which implicates the yhiF gene product in the regulation of C₄-dicarboxylate metabolism. Indeed, when the E. coli C₄-dicarboxylate transporter (encoded by the dctA gene) was expressed in trans, the Suc phenotype of the atp deletion strain reverted to Suc⁺, which shows that the reason why the E. coli atp mutant is unable to grow aerobically on C₄-dicarboxylates is insufficient transport capacity for these substrates.

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^* Corresponding author. Mailing address: Department of Microbiology, Technical University of Denmark, Building 301, DK-2800 Lyngby, Denmark. Phone: 45 45252510. Fax: 45 45932809. E-mail: prj{at}im.dtu.dk.

This paper is dedicated to the memory of Lars Boe.

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Journal of Bacteriology, November 1998, p. 5855-5859, Vol. 180, No. 22
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

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