This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Beach, M. B.
Right arrow Articles by Osuna, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Beach, M. B.
Right arrow Articles by Osuna, R.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 1998, p. 5932-5946, Vol. 180, No. 22
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification and Characterization of the fis Operon in Enteric Bacteria

Michael B. Beach and Robert Osuna*

Department of Biological Sciences, University at Albany, Albany, New York 12222

Received 28 April 1998/Accepted 9 September 1998

The small DNA binding protein Fis is involved in several different biological processes in Escherichia coli. It has been shown to stimulate DNA inversion reactions mediated by the Hin family of recombinases, stimulate integration and excision of phage lambda  genome, regulate the transcription of several different genes including those of stable RNA operons, and regulate the initiation of DNA replication at oriC. fis has also been isolated from Salmonella typhimurium, and the genomic sequence of Haemophilus influenzae reveals its presence in this bacteria. This work extends the characterization of fis to other organisms. Very similar fis operon structures were identified in the enteric bacteria Klebsiella pneumoniae, Serratia marcescens, Erwinia carotovora, and Proteus vulgaris but not in several nonenteric bacteria. We found that the deduced amino acid sequences for Fis are 100% identical in K. pneumoniae, S. marcescens, E. coli, and S. typhimurium and 96 to 98% identical when E. carotovora and P. vulgaris Fis are considered. The deduced amino acid sequence for H. influenzae Fis is about 80% identical and 90% similar to Fis in enteric bacteria. However, in spite of these similarities, the E. carotovora, P. vulgaris, and H. influenzae Fis proteins are not functionally identical. An open reading frame (ORF1) preceding fis in E. coli is also found in all these bacteria, and their deduced amino acid sequences are also very similar. The sequence preceding ORF1 in the enteric bacteria showed a very strong similarity to the E. coli fis P region from -53 to +27 and the region around -116 containing an ihf binding site. Both beta -galactosidase assays and primer extension assays showed that these regions function as promoters in vivo and are subject to growth phase-dependent regulation. However, their promoter strengths vary, as do their responses to Fis autoregulation and integration host factor stimulation.

* Corresponding author. Mailing address: Department of Biological Sciences, 1400 Washington Ave., University at Albany, Albany, NY 12222. Phone: (518) 442-4333. Fax: (518) 442-4767. E-mail: osuna{at}cnsunix.albany.edu.

Journal of Bacteriology, November 1998, p. 5932-5946, Vol. 180, No. 22
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Teras, R., Jakovleva, J., Kivisaar, M. (2009). Fis negatively affects binding of Tn4652 transposase by out-competing IHF from the left end of Tn4652. Microbiology 155: 1203-1214 [Abstract] [Full Text]  
  • Mallik, P., Paul, B. J., Rutherford, S. T., Gourse, R. L., Osuna, R. (2006). DksA Is Required for Growth Phase-Dependent Regulation, Growth Rate-Dependent Control, and Stringent Control of fis Expression in Escherichia coli.. J. Bacteriol. 188: 5775-5782 [Abstract] [Full Text]  
  • Feldman-Cohen, L. S., Shao, Y., Meinhold, D., Miller, C., Colon, W., Osuna, R. (2006). Common and Variable Contributions of Fis Residues to High-Affinity Binding at Different DNA Sequences. J. Bacteriol. 188: 2081-2095 [Abstract] [Full Text]  
  • Walker, K. A., Mallik, P., Pratt, T. S., Osuna, R. (2004). The Escherichia coli fis Promoter Is Regulated by Changes in the Levels of Its Transcription Initiation Nucleotide CTP. J. Biol. Chem. 279: 50818-50828 [Abstract] [Full Text]  
  • Mallik, P., Pratt, T. S., Beach, M. B., Bradley, M. D., Undamatla, J., Osuna, R. (2004). Growth Phase-Dependent Regulation and Stringent Control of fis Are Conserved Processes in Enteric Bacteria and Involve a Single Promoter (fis P) in Escherichia coli. J. Bacteriol. 186: 122-135 [Abstract] [Full Text]  
  • Walker, K. A., Osuna, R. (2002). Factors Affecting Start Site Selection at the Escherichia coli fis Promoter. J. Bacteriol. 184: 4783-4791 [Abstract] [Full Text]  
  • Aiyar, S. E., Gaal, T., Gourse, R. L. (2002). rRNA Promoter Activity in the Fast-Growing Bacterium Vibrio natriegens. J. Bacteriol. 184: 1349-1358 [Abstract] [Full Text]  
  • Vijesurier, R. M., Carlock, L., Blumenthal, R. M., Dunbar, J. C. (2000). Role and Mechanism of Action of C {middle dot} PvuII, a Regulatory Protein Conserved among Restriction-Modification Systems. J. Bacteriol. 182: 477-487 [Abstract] [Full Text]  
  • Walker, K. A., Atkins, C. L., Osuna, R. (1999). Functional Determinants of the Escherichia coli fis Promoter: Roles of -35, -10, and Transcription Initiation Regions in the Response to Stringent Control and Growth Phase-Dependent Regulation. J. Bacteriol. 181: 1269-1280 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»