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 Previous Article

Journal of Bacteriology, November 1998, p. 6072-6075, Vol. 180, No. 22
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Escherichia coli cmlA Gene Encodes the Multidrug Efflux Pump Cmr/MdfA and Is Responsible for Isopropyl-beta -D-Thiogalactopyranoside Exclusion and Spectinomycin Sensitivity

Chantal Bohn and Philippe Bouloc*

Laboratoire des Réseaux de Régulations, Institut de Génétique et Microbiologie, Université Paris-Sud, CNRS/URA2225, F-91405 Orsay Cedex, France

Received 30 June 1998/Accepted 16 September 1998

Expression of cloned genes from isopropyl-beta -D-thiogalactopyranoside (IPTG)-regulated promoters is lowered when the Escherichia coli CmlA/Cmr/MdfA efflux pump is overexpressed, probably due to IPTG exclusion from the cytoplasm. The previously reported cmlA1 mutation confers a similar phenotype. cmlA1 contains an IS30 insertion upstream of cmr/mdfA, which creates a putative promoter. CmlA overproduction also causes spectinomycin hypersensitivity.

* Corresponding author. Mailing address: Laboratoire des Réseaux de Régulations, Institut de Génétique et Microbiologie, Université Paris-Sud, CNRS/URA2225, bâtiment 400, F-91405 Orsay Cedex, France. Phone: (33) 1 69 15 70 16. Fax: (33) 1 69 15 66 78. E-mail: bouloc{at}infobiogen.fr.

Journal of Bacteriology, November 1998, p. 6072-6075, Vol. 180, No. 22
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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