Journal of Bacteriology, November 1998, p. 6072-6075, Vol. 180, No. 22
Laboratoire des Réseaux de
Régulations, Institut de Génétique et
Microbiologie, Université Paris-Sud, CNRS/URA2225, F-91405
Orsay Cedex, France
Received 30 June 1998/Accepted 16 September 1998
Expression of cloned genes from
isopropyl-
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
The Escherichia coli cmlA Gene Encodes the Multidrug
Efflux Pump Cmr/MdfA and Is Responsible for
Isopropyl-
-D-Thiogalactopyranoside Exclusion and
Spectinomycin Sensitivity
-D-thiogalactopyranoside (IPTG)-regulated
promoters is lowered when the Escherichia coli
CmlA/Cmr/MdfA efflux pump is overexpressed, probably due to IPTG
exclusion from the cytoplasm. The previously reported cmlA1
mutation confers a similar phenotype. cmlA1 contains an IS30 insertion upstream of cmr/mdfA, which
creates a putative promoter. CmlA overproduction also causes
spectinomycin hypersensitivity.
*
Corresponding author. Mailing address: Laboratoire des
Réseaux de Régulations, Institut de Génétique
et Microbiologie, Université Paris-Sud, CNRS/URA2225,
bâtiment 400, F-91405 Orsay Cedex, France. Phone: (33) 1 69 15 70 16. Fax: (33) 1 69 15 66 78. E-mail:
bouloc{at}infobiogen.fr.
This article has been cited by other articles:
Copyright © 2009 by the American Society for Microbiology. For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»