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Journal of Bacteriology, December 1998, p. 6107-6116, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Localization of Cell Division Protein FtsQ by Immunofluorescence Microscopy in Dividing and Nondividing Cells of Escherichia coli

Nienke Buddelmeijer,1,dagger Mirjam E. G. Aarsman,1 Arend H. J. Kolk,2 Miguel Vicente,3 and Nanne Nanninga1,*

Institute for Molecular Cell Biology, BioCentrum Amsterdam, University of Amsterdam, 1098 SM Amsterdam,1 and N. H. Swellengrebel Laboratory of Tropical Hygiene, Royal Tropical Institute, 1105 AZ Amsterdam,2 The Netherlands, and Centro de Investigaciones Biológicas, CSIC, 28006 Madrid, Spain3

Received 6 August 1998/Accepted 30 September 1998

The localization of cell division protein FtsQ in Escherichia coli wild-type cells was studied by immunofluorescence microscopy with specific monoclonal antibodies. FtsQ could be localized to the division site in constricting cells. FtsQ could also localize to the division site in ftsQ1(Ts) cells grown at the permissive temperature. A hybrid protein in which the cytoplasmic domain and the transmembrane domain were derived from the gamma  form of penicillin-binding protein 1B and the periplasmic domain was derived from FtsQ was also able to localize to the division site. This result indicates that the periplasmic domain of FtsQ determines the localization of FtsQ, as has also been concluded by others for the periplasmic domain of FtsN. Noncentral FtsQ foci were found in the area of the cell where the nucleoid resides and were therefore assumed to represent sites where the FtsQ protein is synthesized and simultaneously inserted into the cytoplasmic membrane.

* Corresponding author. Mailing address: Institute for Molecular Cell Biology, Kruislaan 316, 1098 SM Amsterdam, The Netherlands. Phone: 31-20-525 5194 or 31-20-525 5187. Fax: 31-20-5256271. E-mail: nanninga{at}bio.uva.nl.

dagger Present address: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA, 02115.

Journal of Bacteriology, December 1998, p. 6107-6116, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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