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Journal of Bacteriology, December 1998, p. 6375-6383, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Two-Component Signal Transduction System Essential for Growth of Bacillus subtilis: Implications for Anti-Infective Therapydagger

Céline Fabret and James A. Hoch*

Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California

Received 24 August 1998/Accepted 25 September 1998

A two-component signal transduction system encoded by the yycF and yycG genes is part of an operon containing three genes, yycH, yycI, and yycJ, with no known function and a gene, yycK, coding for an HtrA-like protease. This operon was transcribed during growth, and its transcription shut down as the cells approached stationary phase. This decreased transcription was not Spo0A dependent. The HtrA protease gene was separately controlled during sporulation from a sigma G promoter. Studies using insertional inactivation plasmids revealed that neither yycF nor yycG could be inactivated, whereas the other genes were inactivated without loss of viability. A temperature-sensitive YycF response regulator mutant was isolated and shown to have an H215P mutation in a putative DNA-binding domain which is closely related to the OmpR family of response regulators. At the nonpermissive temperature, cultures of the mutant strain stopped growth within 30 min, and this was followed by a decrease in optical density. Microscopically, many of the cells appeared to retain their structure while being empty of their contents. The essential processes regulated by this two-component system remain unknown. A search of the genome databases revealed YycF, YycG, and YycJ homologues encoded by three linked genes in Streptococcus pyogenes. The high level of identity of these proteins (71% for YycF) suggests that this system may play a similar role in gram-positive pathogens.

* Corresponding author. Mailing address: Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Rd., La Jolla, CA 92037. Phone: (619) 784-7905. Fax: (619) 784-7966. E-mail: hoch{at}scripps.edu.

dagger Publication 11729-MEM from the Department of Molecular and Experimental Medicine at The Scripps Research Institute.

Journal of Bacteriology, December 1998, p. 6375-6383, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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  • Fukuchi, K., Kasahara, Y., Asai, K., Kobayashi, K., Moriya, S., Ogasawara, N. (2000). The essential two-component regulatory system encoded by yycF and yycG modulates expression of the ftsAZ operon in Bacillus subtilis. Microbiology 146: 1573-1583 [Abstract] [Full Text]  
  • Beier, D., Frank, R. (2000). Molecular Characterization of Two-Component Systems of Helicobacter pylori. J. Bacteriol. 182: 2068-2076 [Abstract] [Full Text]  
  • Noone, D., Howell, A., Devine, K. M. (2000). Expression of ykdA, Encoding a Bacillus subtilis Homologue of HtrA, Is Heat Shock Inducible and Negatively Autoregulated. J. Bacteriol. 182: 1592-1599 [Abstract] [Full Text]  
  • Deschenes, R. J., Lin, H., Ault, A. D., Fassler, J. S. (1999). Antifungal Properties and Target Evaluation of Three Putative Bacterial Histidine Kinase Inhibitors. Antimicrob. Agents Chemother. 43: 1700-1703 [Abstract] [Full Text]  
  • Federle, M. J., McIver, K. S., Scott, J. R. (1999). A Response Regulator That Represses Transcription of Several Virulence Operons in the Group A Streptococcus. J. Bacteriol. 181: 3649-3657 [Abstract] [Full Text]  
  • Martin, P. K., Li, T., Sun, D., Biek, D. P., Schmid, M. B. (1999). Role in Cell Permeability of an Essential Two-Component System in Staphylococcus aureus. J. Bacteriol. 181: 3666-3673 [Abstract] [Full Text]  
  • Fabret, C., Feher, V. A., Hoch, J. A. (1999). Two-Component Signal Transduction in Bacillus subtilis: How One Organism Sees Its World. J. Bacteriol. 181: 1975-1983 [Full Text]  


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