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Journal of Bacteriology, December 1998, p. 6396-6399, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Analysis of a Gene Encoding a Cell-Bound Esterase from Streptomyces chrysomallus

Rico Berger, Matthias Hoffmann,dagger and Ullrich Keller*

Max-Volmer-Institut für Biophysikalische Chemie und Biochemie, Fachgebiet Biochemie und Molekulare Biologie, Technische Universität Berlin, D-10587 Berlin, Germany

Received 18 March 1998/Accepted 11 September 1998

A gene (estA) encoding a 42-kDa cell-bound esterase, EstA, was found to be located 75 bp upstream of the cyclophilin A gene (cypA) of Streptomyces chrysomallus. Western blot analysis revealed the presence of EstA (42 kDa) in cell extracts of S. chrysomallus X2 and Streptomyces lividans. EstA specifically hydrolyzes short-chain p-nitrophenyl esters. EstA formation starts at the end of growth phase, and its activity level remains constant throughout stationary phase. Expression of estA from the melanin (mel) promoter in plasmid pIJ702 led to a substantial increase of total esterase activity in streptomycetes.

* Corresponding author. Mailing address: Max-Volmer-Institut für Biophysikalische Chemie und Biochemie, Fachgebiet Biochemie und Molekulare Biologie, Technische Universität Berlin, Franklinstrasse 29, D-10587 Berlin, Germany. Phone and Fax: 4930 314 73522. E-mail: kellghbe{at}mailszrz.zrz.tu-berlin.de.

dagger Present address: Institut für Bioanalytik, Umwelttoxikologie und Biotechnologie, 06120 Halle, Germany

Journal of Bacteriology, December 1998, p. 6396-6399, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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