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Journal of Bacteriology, December 1998, p. 6429-6432, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

mraY Is an Essential Gene for Cell Growth in Escherichia coli

David S. Boyle* and William D. Donachie

Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland

Received 6 July 1998/Accepted 1 October 1998

The synthesis of the murein precursor lipid I is performed by MraY. We have shown that mraY is an essential gene for cell growth. Cells depleted of MraY first swell and then lyse. The expression of mraY DNA in vitro produces a 40-kDa polypeptide detectable by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.


* Corresponding author. Mailing address: University of Edinburgh, I.C.M.B., Darwin Building, King's Buildings, Mayfield Rd., Edinburgh EH9 3JR, Scotland, United Kingdom. Phone: 44 131 650-5356. Fax: 44 131 668-3870. E-mail: DBOYLE{at}srv0.bio.ed.ac.uk.


Journal of Bacteriology, December 1998, p. 6429-6432, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.



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