This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Germon, P.
Right arrow Articles by Lazzaroni, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Germon, P.
Right arrow Articles by Lazzaroni, J. C.

Next Article 

Journal of Bacteriology, December 1998, p. 6433-6439, Vol. 180, No. 24
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Mutational Analysis of the Escherichia coli K-12 TolA N-Terminal Region and Characterization of Its TolQ-Interacting Domain by Genetic Suppression

Pierre Germon, Thierry Clavel, Anne Vianney, Raymond Portalier, and Jean Claude Lazzaroni*

Laboratoire de Microbiologie et Génétique Moléculaire, CNRS-Université Lyon I, F-69622 Villeurbanne Cedex, France

Received 15 July 1998/Accepted 26 September 1998

The Tol-Pal proteins of Escherichia coli are involved in maintaining outer membrane integrity. They form two complexes in the cell envelope. Transmembrane domains of TolQ, TolR, and TolA interact in the cytoplasmic membrane, while TolB and Pal form a complex near the outer membrane. The N-terminal transmembrane domain of TolA anchors the protein to the cytoplasmic membrane and interacts with TolQ and TolR. Extensive mutagenesis of the N-terminal part of TolA was carried out to characterize the residues involved in such processes. Mutations affecting the function of TolA resulted in a lack or an alteration in TolA-TolQ or TolR-TolA interactions but did not affect the formation of TolQ-TolR complexes. Our results confirmed the importance of residues serine 18 and histidine 22, which are part of an SHLS motif highly conserved in the TolA and the related TonB proteins from different organisms. Genetic suppression experiments were performed to restore the functional activity of some tolA mutants. The suppressor mutations all affected the first transmembrane helix of TolQ. These results confirmed the essential role of the transmembrane domain of TolA in triggering interactions with TolQ and TolR.

* Corresponding author. Mailing address: Laboratoire de Microbiologie et Génétique Moléculaire, CNRS-Université Lyon I, bât. 405, F-69622 Villeurbanne Cedex, France. Phone: (33) 472 43 13 67. Fax: (33) 472 43 19 71. E-mail: lazzaroni{at}biomserv.univ-lyon1.fr.

Journal of Bacteriology, December 1998, p. 6433-6439, Vol. 180, No. 24
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Vankemmelbeke, M., Zhang, Y., Moore, G. R., Kleanthous, C., Penfold, C. N., James, R. (2009). Energy-dependent Immunity Protein Release during tol-dependent Nuclease Colicin Translocation. J. Biol. Chem. 284: 18932-18941 [Abstract] [Full Text]  
  • Zhang, X. Y.-Z., Goemaere, E. L., Thome, R., Gavioli, M., Cascales, E., Lloubes, R. (2009). Mapping the Interactions between Escherichia coli Tol Subunits: ROTATION OF THE TolR TRANSMEMBRANE HELIX. J. Biol. Chem. 284: 4275-4282 [Abstract] [Full Text]  
  • Brinkman, K. K., Larsen, R. A. (2008). Interactions of the Energy Transducer TonB with Noncognate Energy-Harvesting Complexes. J. Bacteriol. 190: 421-427 [Abstract] [Full Text]  
  • Goemaere, E. L., Devert, A., Lloubes, R., Cascales, E. (2007). Movements of the TolR C-terminal Domain Depend on TolQR Ionizable Key Residues and Regulate Activity of the Tol Complex. J. Biol. Chem. 282: 17749-17757 [Abstract] [Full Text]  
  • Larsen, R. A., Deckert, G. E., Kastead, K. A., Devanathan, S., Keller, K. L., Postle, K. (2007). His20 Provides the Sole Functionally Significant Side Chain in the Essential TonB Transmembrane Domain. J. Bacteriol. 189: 2825-2833 [Abstract] [Full Text]  
  • Cascales, E., Buchanan, S. K., Duche, D., Kleanthous, C., Lloubes, R., Postle, K., Riley, M., Slatin, S., Cavard, D. (2007). Colicin Biology. Microbiol. Mol. Biol. Rev. 71: 158-229 [Abstract] [Full Text]  
  • Vianney, A., Jubelin, G., Renault, S., Dorel, C., Lejeune, P., Lazzaroni, J. C. (2005). Escherichia coli tol and rcs genes participate in the complex network affecting curli synthesis. Microbiology 151: 2487-2497 [Abstract] [Full Text]  
  • Llamas, M. A., Rodriguez-Herva, J. J., Hancock, R. E. W., Bitter, W., Tommassen, J., Ramos, J. L. (2003). Role of Pseudomonas putida tol-oprL Gene Products in Uptake of Solutes through the Cytoplasmic Membrane. J. Bacteriol. 185: 4707-4716 [Abstract] [Full Text]  
  • Karlsson, F., Borrebaeck, C. A. K., Nilsson, N., Malmborg-Hager, A.-C. (2003). The Mechanism of Bacterial Infection by Filamentous Phages Involves Molecular Interactions between TolA and Phage Protein 3 Domains. J. Bacteriol. 185: 2628-2634 [Abstract] [Full Text]  
  • Heilpern, A. J., Waldor, M. K. (2003). pIIICTX, a Predicted CTX{phi} Minor Coat Protein, Can Expand the Host Range of Coliphage fd To Include Vibrio cholerae. J. Bacteriol. 185: 1037-1044 [Abstract] [Full Text]  
  • Dubuisson, J. F., Vianney, A., Lazzaroni, J. C. (2002). Mutational Analysis of the TolA C-Terminal Domain of Escherichia coli and Genetic Evidence for an Interaction between TolA and TolB. J. Bacteriol. 184: 4620-4625 [Abstract] [Full Text]  
  • Cascales, E., Bernadac, A., Gavioli, M., Lazzaroni, J.-C., Lloubes, R. (2002). Pal Lipoprotein of Escherichia coli Plays a Major Role in Outer Membrane Integrity. J. Bacteriol. 184: 754-759 [Abstract] [Full Text]  
  • Germon, P., Ray, M.-C., Vianney, A., Lazzaroni, J. C. (2001). Energy-Dependent Conformational Change in the TolA Protein of Escherichia coli Involves Its N-Terminal Domain, TolQ, and TolR. J. Bacteriol. 183: 4110-4114 [Abstract] [Full Text]  
  • Llamas, M. A., Ramos, J. L., Rodríguez-Herva, J. J. (2000). Mutations in Each of the tol Genes of Pseudomonas putida Reveal that They Are Critical for Maintenance of Outer Membrane Stability. J. Bacteriol. 182: 4764-4772 [Abstract] [Full Text]  
  • Heilpern, A. J., Waldor, M. K. (2000). CTXphi Infection of Vibrio cholerae Requires the tolQRA Gene Products. J. Bacteriol. 182: 1739-1747 [Abstract] [Full Text]  
  • Ray, M.-C., Germon, P., Vianney, A., Portalier, R., Lazzaroni, J. C. (2000). Identification by Genetic Suppression of Escherichia coli TolB Residues Important for TolB-Pal Interaction. J. Bacteriol. 182: 821-824 [Abstract] [Full Text]  
  • Bouveret, E., Bénédetti, H., Rigal, A., Loret, E., Lazdunski, C. (1999). In Vitro Characterization of Peptidoglycan-Associated Lipoprotein (PAL)-Peptidoglycan and PAL-TolB Interactions. J. Bacteriol. 181: 6306-6311 [Abstract] [Full Text]  
  • Journet, L., Rigal, A., Lazdunski, C., Bénédetti, H. (1999). Role of TolR N-Terminal, Central, and C-Terminal Domains in Dimerization and Interaction with TolA and TolQ. J. Bacteriol. 181: 4476-4484 [Abstract] [Full Text]  
  • Larsen, R. A., Postle, K. (2001). Conserved Residues Ser16 and His20 and Their Relative Positioning Are Essential for TonB Activity, Cross-linking of TonB with ExbB, and the Ability of TonB to Respond to Proton Motive Force. J. Biol. Chem. 276: 8111-8117 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»