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Journal of Bacteriology, December 1998, p. 6551-6556, Vol. 180, No. 24
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Sequence Diversity of the oprI Gene, Coding for Major Outer Membrane Lipoprotein I, among rRNA Group I Pseudomonads

Daniel De Vos,1,dagger Christiane Bouton,1 Alain Sarniguet,2 Paul De Vos,3 Marc Vauterin,4 and Pierre Cornelis1,*

Laboratory of Microbial Interactions, Department of Immunology, Parasitology, and Ultrastructure,1 and Laboratory of Plant Genetics,4 Flanders Interuniversity Institute of Biotechnology, Vrije Universiteit Brussel, B-1640 Sint Genesius Rode, and Laboratorium voor Microbiologie, Universiteit Gent, B-9000 Ghent,3 Belgium, and INRA, Centre de Recherche de Rennes, Station de Pathologie Végétale, B.P. 26, 35650 Le Rheu, France2

Received 1 June 1998/Accepted 17 September 1998

The sequence of oprI, the gene coding for the major outer membrane lipoprotein I, was determined by PCR sequencing for representatives of 17 species of rRNA group I pseudomonads, with a special emphasis on Pseudomonas aeruginosa and Pseudomonas fluorescens. Within the P. aeruginosa species, oprI sequences for 25 independent isolates were found to be identical, except for one silent substitution at position 96. The oprI sequences diverged more for the other rRNA group I pseudomonads (85 to 91% similarity with P. aeruginosa oprI). An accumulation of silent and also (but to a much lesser extent) nonsilent substitutions in the different sequences was found. A clustering according to the respective presence and/or positions of the HaeIII, PvuII, and SphI sites could also be obtained. A sequence cluster analysis showed a rather widespread distribution of P. fluorescens isolates. All other rRNA group I pseudomonads clustered in a manner that was in agreement with other studies, showing that the oprI gene can be useful as a complementary phylogenetic marker for classification of rRNA group I pseudomonads.

* Corresponding author. Mailing address: Department of Immunology, Parasitology, and Ultrastructure, Flanders Interuniversity Institute of Biotechnology, Vrije Universiteit Brussel, Paardenstraat 65, B-1640 Sint Genesius Rode, Belgium. Phone: 32 2 3590221. Fax: 32 2 3590390. E-mail: pcornel{at}pop.vub.ac.be.

dagger Present address: INNOGENETICS, B-1120 Neder-Over-Heembeek, Belgium.

Journal of Bacteriology, December 1998, p. 6551-6556, Vol. 180, No. 24
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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