This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kilburn, L.
Right arrow Articles by Neshat, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kilburn, L.
Right arrow Articles by Neshat, S.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 1998, p. 6753-6756, Vol. 180, No. 24
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Insertion Mutagenesis of the Ferric Pyoverdine Receptor FpvA of Pseudomonas aeruginosa: Identification of Permissive Sites and a Region Important for Ligand Binding

Laurie Kilburn,1 Keith Poole,1,* Jean-Marie Meyer,2 and Shadi Neshat1

Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada,1 and Laboratoire de Microbiologie et de Génétique, Unité de Recherche Associée au Centre National de la Recherche Scientifique No. 1481, Université Louis-Pasteur, 67000 Strasbourg, France2

Received 3 September 1998/Accepted 8 October 1998

Insertion of an 18-amino-acid-encoding sequence within the fpvA gene identified permissive sites at residues Y350, A402, R451, R521, and R558, consistent with these residues occurring in extramembranous loop regions of the protein. Insertions at R451, R521, and R558 did not adversely affect receptor function, although insertions at Y350 and A402 compromised ferric pyoverdine binding and uptake. The latter region likely contributes to or interacts with the ligand-binding site.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada. Phone: (613) 545-6677. Fax: (613) 545-6796. E-mail: poolek{at}post.queensu.ca.

Journal of Bacteriology, December 1998, p. 6753-6756, Vol. 180, No. 24
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Shen, J.-S., Geoffroy, V., Neshat, S., Jia, Z., Meldrum, A., Meyer, J.-M., Poole, K. (2005). FpvA-Mediated Ferric Pyoverdine Uptake in Pseudomonas aeruginosa: Identification of Aromatic Residues in FpvA Implicated in Ferric Pyoverdine Binding and Transport. J. Bacteriol. 187: 8511-8515 [Abstract] [Full Text]  
  • James, H. E., Beare, P. A., Martin, L. W., Lamont, I. L. (2005). Mutational Analysis of a Bifunctional Ferrisiderophore Receptor and Signal-Transducing Protein from Pseudomonas aeruginosa. J. Bacteriol. 187: 4514-4520 [Abstract] [Full Text]  
  • Shen, J., Meldrum, A., Poole, K. (2002). FpvA Receptor Involvement in Pyoverdine Biosynthesis in Pseudomonas aeruginosa. J. Bacteriol. 184: 3268-3275 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»