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Journal of Bacteriology, December 1998, p. 6757-6760, Vol. 180, No. 24
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The aadA Gene of Plasmid R100 Confers Resistance to Spectinomycin and Streptomycin in Myxococcus xanthus

Vincent Magrini, Chad Creighton, David White, Patricia L. Hartzell, and Philip Youderian*

Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, Idaho 83844-3052

Received 1 May 1998/Accepted 2 October 1998

Plasmids with the aadA gene from plasmid R100, which confers resistance to the aminoglycosides spectinomycin and streptomycin in Escherchia coli, can be introduced into wild-type Myxococcus xanthus, strain DK1622, by electroporation. Recombinant M. xanthus strains with integrated plasmids carrying the aadA gene acquire resistance to high levels of these antibiotics. Selection for aadA in M. xanthus can be carried out independently of, or simultaneously with, selection for resistance to kanamycin. The kinds and frequencies of recombination events observed between integrative plasmids with aadA and the M. xanthus chromosome are similar to those observed after the transformation of yeast. Cleavage of integrative plasmid DNA at a site adjacent to a region of homology between the plasmid and the M. xanthus genome favors the targeted disruption of M. xanthus genes by allele replacement.

* Corresponding author. Mailing address: Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, ID 83844-3052. Phone: (208) 885-0571. Fax: (208) 885-6518. E-mail: pay{at}uidaho.edu.

Journal of Bacteriology, December 1998, p. 6757-6760, Vol. 180, No. 24
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Frank, K. L., Bundle, S. F., Kresge, M. E., Eggers, C. H., Samuels, D. S. (2003). aadA Confers Streptomycin Resistance in Borrelia burgdorferi. J. Bacteriol. 185: 6723-6727 [Abstract] [Full Text]  
  • Magrini, V., Creighton, C., Youderian, P. (1999). Site-Specific Recombination of Temperate Myxococcus xanthus Phage Mx8: Genetic Elements Required for Integration. J. Bacteriol. 181: 4050-4061 [Abstract] [Full Text]  
  • Magrini, V., Storms, M. L., Youderian, P. (1999). Site-Specific Recombination of Temperate Myxococcus xanthus Phage Mx8: Regulation of Integrase Activity by Reversible, Covalent Modification. J. Bacteriol. 181: 4062-4070 [Abstract] [Full Text]  


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