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Journal of Bacteriology, December 1998, p. 6769-6772, Vol. 180, No. 24
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Active Efflux of Organic Solvents by Pseudomonas putida S12 Is Induced by Solvents

Jasper Kieboom,1,* Jonathan J. Dennis,2 Gerben J. Zylstra,2 and Jan A. M. de Bont1

Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen Agricultural University, Wageningen, The Netherlands,1 and Biotechnology Center for Agriculture and the Environment, Rutgers University, New Brunswick, New Jersey 08901-85202

Received 16 July 1998/Accepted 7 October 1998

Induction of the membrane-associated organic solvent efflux system SrpABC of Pseudomonas putida S12 was examined by cloning a 312-bp DNA fragment, containing the srp promoter, in the broad-host-range reporter vector pKRZ-1. Compounds that are capable of inducing expression of the srpABC genes include aromatic and aliphatic solvents and alcohols. General stress conditions such as pH, temperature, NaCl, or the presence of organic acids did not induce srp transcription. Although the solvent efflux pump in P. putida S12 is a member of the resistance-nodulation-cell division family of transporters, the srpABC genes were not induced by antibiotics or heavy metals.

* Corresponding author. Mailing address: Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, P.O. Box 8129, 6700 EV Wageningen, The Netherlands. Phone: 31 (0)317 484412. Fax: 31 (0)317 484978. E-mail: jasper.kieboom{at}imb.ftns.wau.nl.

Journal of Bacteriology, December 1998, p. 6769-6772, Vol. 180, No. 24
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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