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Journal of Bacteriology, December 1998, p. 6776-6779, Vol. 180, No. 24
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Sucrose-Phosphate Synthase from Synechocystis sp. Strain PCC 6803: Identification of the spsA Gene and Characterization of the Enzyme Expressed in Escherichia coli

Leonardo Curatti,1 Eduardo Folco,1 Paula Desplats,1 Gustavo Abratti,1 Verónica Limones,2 Luis Herrera-Estrella,2 and Graciela Salerno1,*

Centro de Investigaciones Biológicas, Fundación para Investigaciones Biológicas Aplicadas (FIBA) and PROBIOP-CONICET, 7600 Mar del Plata, Argentina,1 and Departamento de Ingeniería Genética de Plantas, Centro de Investigación y Estudios Avanzados del IPN, Unidad Irapuato, Irapuato, Gto., México2

Received 29 July 1998/Accepted 8 October 1998

The first identification and characterization of a prokaryotic gene (spsA) encoding sucrose-phosphate synthase (SPS) is reported for Synechocystis sp. strain PCC 6803, a unicellular non-nitrogen-fixing cyanobacterium. Comparisons of the deduced amino acid sequence and some relevant biochemical properties of the enzyme with those of plant SPSs revealed important differences in the N-terminal and UDP-glucose binding site regions, substrate specificities, molecular masses, subunit compositions, and regulatory properties.

* Corresponding author. Mailing address: Centro de Investigaciones Biológicas (FIBA), Casilla de Correo 1348, 7600 Mar del Plata, Argentina. Phone: (54) 23 748257. FAX: (54) 23 757120. E-mail: fiba{at}mdq.com.ar.

Journal of Bacteriology, December 1998, p. 6776-6779, Vol. 180, No. 24
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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