This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wösten, M. M. S. M.
Right arrow Articles by van der Zeijst, B. A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wösten, M. M. S. M.
Right arrow Articles by van der Zeijst, B. A. M.

 Previous Article  |  Next Article 

J Bacteriol, February 1998, p. 594-599, Vol. 180, No. 3
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of Campylobacter jejuni Promoter Sequences

Marc M. S. M. Wösten, Miranda Boeve, Mirjam G. A. Koot, Ad C. van Nuenen, and Bernard A. M. van der Zeijst*

Department of Bacteriology, Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Universiteit Utrecht, 3508 TD Utrecht, The Netherlands

Received 10 September 1997/Accepted 4 December 1997

A promoterless lacZ shuttle vector, which allowed screening of promoters by beta -galactosidase activity in Campylobacter jejuni and Escherichia coli, was developed. Chromosomal DNA fragments from C. jejuni were cloned into this vector; 125 of 1,824 clones displayed promoter activity in C. jejuni. Eleven clones with strong promoter activity in C. jejuni were further characterized. Their nucleotide sequences were determined, and the transcriptional start sites of the putative promoters in C. jejuni were determined by primer extension. Only 6 of these 11 promoters were functional in E. coli. The 11 newly characterized and 10 previously characterized C. jejuni promoters were used to establish a consensus sequence for C. jejuni promoters. The 21 promoters were found to be very similar. They contain three conserved regions, located approximately 10, 16, and 35 bp upstream of the transcriptional start point. The -10 region resembles that of a typical sigma 70 E. coli promoter, but the -35 region is completely different. In addition a -16 region typical for gram-positive bacteria was identified.

* Corresponding author. Mailing address: Department of Bacteriology, Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, P.O. Box 80.165, 3508 TD Utrecht, The Netherlands. Phone: 31-30-2534344. Fax: 31-30-2540784. E-mail: Zeijst{at}vetmic.dgk.ruu.nl.




This article has been cited by other articles:

  • Wang, L., Jeon, B., Sahin, O., Zhang, Q. (2009). Identification of an Arsenic Resistance and Arsenic-Sensing System in Campylobacter jejuni. Appl. Environ. Microbiol. 75: 5064-5073 [Abstract] [Full Text]  
  • Jeon, B., Muraoka, W., Scupham, A., Zhang, Q. (2009). Roles of lipooligosaccharide and capsular polysaccharide in antimicrobial resistance and natural transformation of Campylobacter jejuni. J Antimicrob Chemother 63: 462-468 [Abstract] [Full Text]  
  • Jeon, B., Muraoka, W., Sahin, O., Zhang, Q. (2008). Role of Cj1211 in Natural Transformation and Transfer of Antibiotic Resistance Determinants in Campylobacter jejuni. Antimicrob. Agents Chemother. 52: 2699-2708 [Abstract] [Full Text]  
  • Malik-Kale, P., Parker, C. T., Konkel, M. E. (2008). Culture of Campylobacter jejuni with Sodium Deoxycholate Induces Virulence Gene Expression. J. Bacteriol. 190: 2286-2297 [Abstract] [Full Text]  
  • Guo, B., Wang, Y., Shi, F., Barton, Y.-W., Plummer, P., Reynolds, D. L., Nettleton, D., Grinnage-Pulley, T., Lin, J., Zhang, Q. (2008). CmeR Functions as a Pleiotropic Regulator and Is Required for Optimal Colonization of Campylobacter jejuni In Vivo. J. Bacteriol. 190: 1879-1890 [Abstract] [Full Text]  
  • Miller, C. E., Rock, J. D., Ridley, K. A., Williams, P. H., Ketley, J. M. (2008). Utilization of Lactoferrin-Bound and Transferrin-Bound Iron by Campylobacter jejuni. J. Bacteriol. 190: 1900-1911 [Abstract] [Full Text]  
  • Jeon, B., Zhang, Q. (2007). Cj0011c, a Periplasmic Single- and Double-Stranded DNA-Binding Protein, Contributes to Natural Transformation in Campylobacter jejuni. J. Bacteriol. 189: 7399-7407 [Abstract] [Full Text]  
  • Kamal, N., Dorrell, N., Jagannathan, A., Turner, S. M., Constantinidou, C., Studholme, D. J., Marsden, G., Hinds, J., Laing, K. G., Wren, B. W., Penn, C. W. (2007). Deletion of a previously uncharacterized flagellar-hook-length control gene fliK modulates the {sigma}54-dependent regulon in Campylobacter jejuni. Microbiology 153: 3099-3111 [Abstract] [Full Text]  
  • Chen, S., Bagdasarian, M., Kaufman, M. G., Bates, A. K., Walker, E. D. (2007). Mutational Analysis of the ompA Promoter from Flavobacterium johnsoniae. J. Bacteriol. 189: 5108-5118 [Abstract] [Full Text]  
  • Kienesberger, S., Gorkiewicz, G., Joainig, M. M., Scheicher, S. R., Leitner, E., Zechner, E. L. (2007). Development of Experimental Genetic Tools for Campylobacter fetus. Appl. Environ. Microbiol. 73: 4619-4630 [Abstract] [Full Text]  
  • Malik-Kale, P., Raphael, B. H., Parker, C. T., Joens, L. A., Klena, J. D., Quinones, B., Keech, A. M., Konkel, M. E. (2007). Characterization of Genetically Matched Isolates of Campylobacter jejuni Reveals that Mutations in Genes Involved in Flagellar Biosynthesis Alter the Organism's Virulence Potential. Appl. Environ. Microbiol. 73: 3123-3136 [Abstract] [Full Text]  
  • Thompson, S. A., Maani, E. V., Lindell, A. H., King, C. J., McArthur, J. V. (2007). Novel Tetracycline Resistance Determinant Isolated from an Environmental Strain of Serratia marcescens. Appl. Environ. Microbiol. 73: 2199-2206 [Abstract] [Full Text]  
  • Ashgar, S. S. A., Oldfield, N. J., Wooldridge, K. G., Jones, M. A., Irving, G. J., Turner, D. P. J., Ala'Aldeen, D. A. A. (2007). CapA, an Autotransporter Protein of Campylobacter jejuni, Mediates Association with Human Epithelial Cells and Colonization of the Chicken Gut. J. Bacteriol. 189: 1856-1865 [Abstract] [Full Text]  
  • Chen, S., Bagdasarian, M., Kaufman, M. G., Walker, E. D. (2007). Characterization of Strong Promoters from an Environmental Flavobacterium hibernum Strain by Using a Green Fluorescent Protein-Based Reporter System. Appl. Environ. Microbiol. 73: 1089-1100 [Abstract] [Full Text]  
  • Ridley, K. A., Rock, J. D., Li, Y., Ketley, J. M. (2006). Heme Utilization in Campylobacter jejuni. J. Bacteriol. 188: 7862-7875 [Abstract] [Full Text]  
  • Akiba, M., Lin, J., Barton, Y.-W., Zhang, Q. (2006). Interaction of CmeABC and CmeDEF in conferring antimicrobial resistance and maintaining cell viability in Campylobacter jejuni. J Antimicrob Chemother 57: 52-60 [Abstract] [Full Text]  
  • Lin, J., Cagliero, C., Guo, B., Barton, Y.-W., Maurel, M.-C., Payot, S., Zhang, Q. (2005). Bile Salts Modulate Expression of the CmeABC Multidrug Efflux Pump in Campylobacter jejuni. J. Bacteriol. 187: 7417-7424 [Abstract] [Full Text]  
  • Karlyshev, A. V., Wren, B. W. (2005). Development and Application of an Insertional System for Gene Delivery and Expression in Campylobacter jejuni. Appl. Environ. Microbiol. 71: 4004-4013 [Abstract] [Full Text]  
  • Lin, J., Akiba, M., Sahin, O., Zhang, Q. (2005). CmeR Functions as a Transcriptional Repressor for the Multidrug Efflux Pump CmeABC in Campylobacter jejuni. Antimicrob. Agents Chemother. 49: 1067-1075 [Abstract] [Full Text]  
  • Carrillo, C. D., Taboada, E., Nash, J. H. E., Lanthier, P., Kelly, J., Lau, P. C., Verhulp, R., Mykytczuk, O., Sy, J., Findlay, W. A., Amoako, K., Gomis, S., Willson, P., Austin, J. W., Potter, A., Babiuk, L., Allan, B., Szymanski, C. M. (2004). Genome-wide Expression Analyses of Campylobacter jejuni NCTC11168 Reveals Coordinate Regulation of Motility and Virulence by flhA. J. Biol. Chem. 279: 20327-20338 [Abstract] [Full Text]  
  • Wosten, M. M. S. M., Wagenaar, J. A., van Putten, J. P. M. (2004). The FlgS/FlgR Two-component Signal Transduction System Regulates the fla Regulon in Campylobacter jejuni. J. Biol. Chem. 279: 16214-16222 [Abstract] [Full Text]  
  • Mixter, P. F., Klena, J. D., Flom, G. A., Siegesmund, A. M., Konkel, M. E. (2003). In Vivo Tracking of Campylobacter jejuni by Using a Novel Recombinant Expressing Green Fluorescent Protein. Appl. Environ. Microbiol. 69: 2864-2874 [Abstract] [Full Text]  
  • Matz, C., van Vliet, A. H. M., Ketley, J. M., Penn, C. W. (2002). Mutational and transcriptional analysis of the Campylobacter jejuni flagellar biosynthesis gene flhB. Microbiology 148: 1679-1685 [Abstract] [Full Text]  
  • Golden, N. J., Acheson, D. W. K. (2002). Identification of Motility and Autoagglutination Campylobacter jejuni Mutants by Random Transposon Mutagenesis. Infect. Immun. 70: 1761-1771 [Abstract] [Full Text]  
  • Matsheka, M. I., Lastovica, A. J., Elisha, B. G. (2001). Molecular Identification of Campylobacter concisus. J. Clin. Microbiol. 39: 3684-3689 [Abstract] [Full Text]  
  • Doree, S. M., Mulks, M. H. (2001). Identification of an Actinobacillus pleuropneumoniae Consensus Promoter Structure. J. Bacteriol. 183: 1983-1989 [Abstract] [Full Text]  
  • Miller, W. G., Bates, A. H., Horn, S. T., Brandl, M. T., Wachtel, M. R., Mandrell, R. E. (2000). Detection on Surfaces and in Caco-2 Cells of Campylobacter jejuni Cells Transformed with New gfp, yfp, and cfp Marker Plasmids. Appl. Environ. Microbiol. 66: 5426-5436 [Abstract] [Full Text]  
  • Day, W. A. Jr., Sajecki, J. L., Pitts, T. M., Joens, L. A. (2000). Role of Catalase in Campylobacter jejuni Intracellular Survival. Infect. Immun. 68: 6337-6345 [Abstract] [Full Text]  
  • Golden, N. J., Camilli, A., Acheson, D. W. K. (2000). Random Transposon Mutagenesis of Campylobacter jejuni. Infect. Immun. 68: 5450-5453 [Abstract] [Full Text]  
  • Baillon, M.-L. A., van Vliet, A. H. M., Ketley, J. M., Constantinidou, C., Penn, C. W. (1999). An Iron-Regulated Alkyl Hydroperoxide Reductase (AhpC) Confers Aerotolerance and Oxidative Stress Resistance to the Microaerophilic Pathogen Campylobacter jejuni. J. Bacteriol. 181: 4798-4804 [Abstract] [Full Text]  
  • Forsyth, M. H., Cover, T. L. (1999). Mutational Analysis of the vacA Promoter Provides Insight into Gene Transcription in Helicobacter pylori. J. Bacteriol. 181: 2261-2266 [Abstract] [Full Text]  
  • Thies, F. L., Karch, H., Hartung, H.-P., Giegerich, G. (1999). Cloning and Expression of the dnaK Gene of Campylobacter jejuni and Antigenicity of Heat Shock Protein 70. Infect. Immun. 67: 1194-1200 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»