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J Bacteriol, February 1998, p. 746-748, Vol. 180, No. 3
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A New Transformation-Deficient Mutant of Haemophilus influenzae Rd with Normal DNA Uptake

M. L. Gwinn,* R. Ramanathan, H. O. Smith, and J.-F. Tombdagger

Department of Molecular Biology and Genetics, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21205

Received 8 May 1997/Accepted 17 November 1997

Haemophilus influenzae Rd is a gram-negative natural transformer. A mutant strain, RJ248, that has normal DNA uptake and translocation but whose transformation frequency is 300 times lower than that of wild-type H. influenzae and whose phage recombination is 8 times lower was isolated. The affected gene, comM, is induced during competence development in wild-type H. influenzae but not in RJ248.

* Corresponding author. Mailing address: The Institute for Genomic Research, 9712 Medical Center Dr., Rockville, MD 20850. Phone: (301) 315-2536. Fax: (301) 838-0208. E-mail: mlgwinn{at}tigr.org.

dagger Present address: The Institute for Genomic Research, Rockville, MD 20850.




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