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J Bacteriol, February 1998, p. 901-908, Vol. 180, No. 4
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Ordered Cloned DNA Map of the Genome of Vibrio cholerae 569B and Localization of Genetic Markers

Soma Chatterjee, Asim K. Mondal,dagger Nasim A. Begum, Susanta Roychoudhury,* and Jyotirmoy Das

Biophysics Division, Indian Institute of Chemical Biology, Calcutta 700 032, India

Received 25 July 1997/Accepted 6 December 1997

By using a low-resolution macrorestriction map as the foundation (R. Majumder et al., J. Bacteriol. 176:1105-1112, 1996), an ordered cloned DNA map of the 3.2-Mb chromosome of the hypertoxinogenic strain 569B of Vibrio cholerae has been constructed. A cosmid library the size of about 4,000 clones containing more than 120 Mb of V. cholerae genomic DNA (40-genome equivalent) was generated. By combining landmark analysis and chromosome walking, the cosmid clones were assembled into 13 contigs covering about 90% of the V. cholerae genome. A total of 92 cosmid clones were assigned to the genome and to regions defined by NotI, SfiI, and CeuI macrorestriction maps. Twenty-seven cloned genes, 9 rrn operons, and 10 copies of a repetitive DNA sequence (IS1004) have been positioned on the ordered cloned DNA map.

* Corresponding author. Mailing address: Biophysics Division, Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Rd., Calcutta 700 032, India. Phone: 91-33-473 0350/5197/5368. Fax: 91-33-473 0350/5197/0284. E-mail: biophy{at}cal.vsnl.net.in.

dagger Present address: Heritable Disorder Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.




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