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J Bacteriol, March 1998, p. 1103-1109, Vol. 180, No. 5
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cloning and Characterization of Transcription of the xylAB Operon in Thermoanaerobacter ethanolicusdagger

Milutin Erbeznik, Karl A. Dawson, and Herbert J. Strobel*

Department of Animal Sciences, University of Kentucky, Lexington, Kentucky 40546-0215

Received 18 August 1997/Accepted 23 December 1997

The genes encoding xylose isomerase (xylA) and xylulose kinase (xylB) from the thermophilic anaerobe Thermoanaerobacter ethanolicus were found to constitute an operon with the transcription initiation site 169 nucleotides upstream from the previously assigned (K. Dekker, H. Yamagata, K. Sakaguchi, and S. Udaka, Agric. Biol. Chem. 55:221-227, 1991) promoter region. The bicistronic xylAB mRNA was processed by cleavage within the 5'-terminal portion of the XylB-coding sequence. Transcription of xylAB was induced in the presence of xylose, and, unlike in all other xylose-utilizing bacteria studied, was not repressed by glucose. The existence of putative xyl operator sequences suggested that xylose utilization is controlled by a repressor-operator mechanism. The T. ethanolicus xylB gene coded for a 500-amino-acid-residue protein with a deduced amino acid sequence highly homologous to those of other XylBs. This is the first report of an xylB nucleotide sequence and an xylAB operon from a thermophilic anaerobic bacterium.

* Corresponding author. Mailing address: 212 W. P. Garrigus Building, Department of Animal Sciences, University of Kentucky, Lexington, KY 40546-0215. Phone: (606) 257-7554. Fax: (606) 257-5318. E-mail: strobel{at}pop.uky.edu.

dagger Published with the approval of the Director of the Kentucky Agricultural Experiment Station as journal article no. 98-07-12.




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