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J Bacteriol, March 1998, p. 1110-1118, Vol. 180, No. 5
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Variation in Flagellin Genes and Proteins of Burkholderia cepacia

Barbara A. Hales,1 J. Alun W. Morgan,2 C. Anthony Hart,3 and Craig Winstanley1,*

Department of Biomedical Sciences, University of Bradford, Bradford, West Yorkshire BD7 1DP,1 Horticulture Research International, Wellesbourne, Warwickshire CV35 9EF,2 and Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, Liverpool L69 3BX,3 United Kingdom

Received 15 August 1997/Accepted 26 December 1997

The majority of isolates of Burkholderia cepacia, an important opportunistic pathogen associated with cystic fibrosis, can be classified into two types on the basis of flagellin protein size. Electron microscopic analysis indicates that the flagella of strains with the larger flagellin type (type I) are wider in diameter. Flagellin genes representative of both types were cloned and sequenced to design oligonucleotide primers for PCR amplification of the central variable domain of B. cepacia flagellin genes. PCR-restriction fragment length polymorphism analysis of amplified B. cepacia flagellin gene products from 16 strains enabled flagellin type classification on the basis of product size and revealed considerable differences in sequence, indicating that the flagellin gene is a useful biomarker for epidemiological and phylogenetic studies of this organism.

* Corresponding author. Mailing address: Department of Biomedical Sciences, University of Bradford, Bradford, West Yorkshire BD7 1DP, United Kingdom. Phone: 44 1274 383561. Fax: 44 1274 309742. E-mail: C.Winstanley{at}bradford.ac.uk.




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