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J Bacteriol, March 1998, p. 1446-1453, Vol. 180, No. 6
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

IroN, a Novel Outer Membrane Siderophore Receptor Characteristic of Salmonella enterica

Andreas J. Bäumler,1,* Tracy L. Norris,1 Todd Lasco,1 Wolfgang Voigt,2 Rolf Reissbrodt,2 Wolfgang Rabsch,3 and Fred Heffron4

Department of Medical Microbiology and Immunology, Texas A&M University, College Station, Texas 77843-11141; Robert Koch-Institut2 and Bundesinstitut für Gesundheitlichen Verbraucherschutz und Veterinärmedizin,3 38843 Wernigerode, Germany; and Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201-30984

Received 3 October 1997/Accepted 6 January 1998

Speciation in enterobacteria involved horizontal gene transfer. Therefore, analysis of genes acquired by horizontal transfer that are present in one species but not its close relatives is expected to give insights into how new bacterial species were formed. In this study we characterize iroN, a gene located downstream of the iroBC operon in the iroA locus of Salmonella enterica serotype Typhi. Like iroBC, the iroN gene is present in all phylogenetic lineages of S. enterica but is absent from closely related species such as Salmonella bongori or Escherichia coli. Comparison of the deduced amino acid sequence of iroN with other proteins suggested that this gene encodes an outer membrane siderophore receptor protein. Mutational analysis in S. enterica and expression in E. coli identified a 78-kDa outer membrane protein as the iroN gene product. When introduced into an E. coli fepA cir fiu aroB mutant on a cosmid, iroN mediated utilization of structurally related catecholate siderophores, including N-(2,3-dihydroxybenzoyl)-L-serine, myxochelin A, benzaldehyde-2,3-dihydroxybenzhydrazone, 2-N,6-N-bis(2,3-dihydroxybenzoyl)-L-lysine, 2-N,6-N-bis(2,3-dihydroxybenzoyl)-L-lysine amide, and enterochelin. These results suggest that the iroA locus functions in iron acquisition in S. enterica.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, Texas A&M University, 407 Reynolds Medical Building, College Station, TX 77843-1114. Phone: (409) 862-7756. Fax: (409) 845-3479. E-mail: abaumler{at}tamu.edu.




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