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J Bacteriol, March 1998, p. 1496-1503, Vol. 180, No. 6
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Role of the fnrL Gene in Photosystem Gene Expression and Photosynthetic Growth of Rhodobacter sphaeroides 2.4.1

Jill H. Zeilstra-Ryalls and Samuel Kaplan^*

Department of Microbiology and Molecular Genetics, University of Texas Health Science CenterHouston, Houston, Texas 77030

Received 21 August 1997/Accepted 3 January 1998

Anoxygenic photosynthetic growth of Rhodobacter sphaeroides 2.4.1 requires a functional fnrL gene, which encodes the anaerobic regulator, FnrL. Using transcriptional fusions to the puc operon in which the upstream FNR consensus-like sequence is either present or absent, we obtained results that suggest that FnrL has both a direct and an indirect role in puc operon expression. In addition to FnrL, several other factors, including the two-component Prr regulatory system and the transcriptional repressor PpsR, are known to mediate oxygen control of photosynthesis gene expression in this organism. Therefore, we examined the relationship between FnrL and these other regulatory elements. Our results indicate that while mutations of prr or ppsR can lead to an increase in expression of some photosynthesis genes under aerobic and anaerobic conditions, regardless of the presence or absence of FnrL, there remains an absolute requirement for a functional fnrL gene for photosynthetic growth. We examined the potential role(s) of FnrL in photosynthetic growth by considering several target genes which may be required for this growth mode.

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^* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, University of Texas Health Science CenterHouston, 6431 Fannin St., Houston, TX 77030. Phone: (713) 500-5502. Fax: (713) 500-5499. E-mail: skaplan{at}utmmg.med.uth.tmc.edu.

Permanent address: Department of Biological Sciences, Oakland University, Rochester, MI 48309.

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