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J Bacteriol, March 1998, p. 1582-1585, Vol. 180, No. 6
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Isolation and Purification of Two Novel Streptomycete RNase Inhibitors, SaI14 and SaI20, and Cloning, Sequencing, and Expression in Escherichia coli of the Gene Coding for SaI14

Daniela Krajcikova,1,* Robert W. Hartley,2 and Jozef Sevcik1

Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia,1 and Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 208922

Received 2 October 1997/Accepted 15 January 1998

Two new RNase inhibitors, SaI14 (Mr, ~14,000) and SaI20 (Mr, ~20,000), were isolated and purified from a Streptomyces aureofaciens strain. The gene sai14, coding for SaI14 protein, was cloned and expressed in Escherichia coli. The alignment of the deduced amino acid sequence of SaI14 with that of barstar, the RNase inhibitor from Bacillus amyloliquefaciens, showed significant similarity between them, especially in the region which contains most of the residues involved in barnase-barstar complex formation.

* Corresponding author. Mailing address: Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, 842 51 Bratislava, Slovakia. Phone: 4217-378-2426. Fax: 4217-372316. E-mail: umbidana{at}savba.savba.sk.






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