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J Bacteriol, March 1998, p. 1596-1599, Vol. 180, No. 6
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Cloning and Sequencing of a Form II
Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from the Bacterial
Symbiont of the Hydrothermal Vent Tubeworm Riftia
pachyptila
Jonathan J.
Robinson,1
Jeffrey L.
Stein,2 and
Colleen M.
Cavanaugh1,*
Department of Organismic and Evolutionary
Biology, Harvard University, Cambridge, Massachusetts
02138,1 and
Diversa Corp., San Diego,
California 921212
Received 4 August 1997/Accepted 13 January 1998
The bacterial symbiont of the hydrothermal vent tubeworm fixes
carbon via the Calvin-Benson cycle and has been shown previously to
express a form II ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO). The gene cbbM, which encodes this enzyme, has
been cloned and sequenced. The gene has the highest identity with the cbbM gene from Rhodospirillum rubrum, and
analysis of the inferred amino acid sequence reveals that all
active-site residues are conserved. This is the first form II RubisCO
cloned and sequenced from a chemoautotrophic symbiont and from a
deep-sea organism.
*
Corresponding author. Mailing address: Department of
Organismic and Evolutionary Biology, Harvard University, 16 Divinity Ave., Cambridge, MA 02138. Phone: 617-495-2177. Fax: 617-496-6933. E-mail: cavanaug{at}hump.harvard.edu.
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