This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Robinson, J. J.
Right arrow Articles by Cavanaugh, C. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Robinson, J. J.
Right arrow Articles by Cavanaugh, C. M.

 Previous Article  |  Next Article 

J Bacteriol, March 1998, p. 1596-1599, Vol. 180, No. 6
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cloning and Sequencing of a Form II Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from the Bacterial Symbiont of the Hydrothermal Vent Tubeworm Riftia pachyptila

Jonathan J. Robinson,1 Jeffrey L. Stein,2 and Colleen M. Cavanaugh1,*

Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138,1 and Diversa Corp., San Diego, California 921212

Received 4 August 1997/Accepted 13 January 1998

The bacterial symbiont of the hydrothermal vent tubeworm fixes carbon via the Calvin-Benson cycle and has been shown previously to express a form II ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO). The gene cbbM, which encodes this enzyme, has been cloned and sequenced. The gene has the highest identity with the cbbM gene from Rhodospirillum rubrum, and analysis of the inferred amino acid sequence reveals that all active-site residues are conserved. This is the first form II RubisCO cloned and sequenced from a chemoautotrophic symbiont and from a deep-sea organism.

* Corresponding author. Mailing address: Department of Organismic and Evolutionary Biology, Harvard University, 16 Divinity Ave., Cambridge, MA 02138. Phone: 617-495-2177. Fax: 617-496-6933. E-mail: cavanaug{at}hump.harvard.edu.




This article has been cited by other articles:

  • Tolli, J., King, G. M. (2005). Diversity and Structure of Bacterial Chemolithotrophic Communities in Pine Forest and Agroecosystem Soils. Appl. Environ. Microbiol. 71: 8411-8418 [Abstract] [Full Text]  
  • Handelsman, J. (2004). Metagenomics: Application of Genomics to Uncultured Microorganisms. Microbiol. Mol. Biol. Rev. 68: 669-685 [Abstract] [Full Text]  
  • Campbell, B. J., Cary, S. C. (2004). Abundance of Reverse Tricarboxylic Acid Cycle Genes in Free-Living Microorganisms at Deep-Sea Hydrothermal Vents. Appl. Environ. Microbiol. 70: 6282-6289 [Abstract] [Full Text]  
  • Giri, B. J., Bano, N., Hollibaugh, J. T. (2004). Distribution of RuBisCO Genotypes along a Redox Gradient in Mono Lake, California. Appl. Environ. Microbiol. 70: 3443-3448 [Abstract] [Full Text]  
  • Nanba, K., King, G. M., Dunfield, K. (2004). Analysis of Facultative Lithotroph Distribution and Diversity on Volcanic Deposits by Use of the Large Subunit of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase. Appl. Environ. Microbiol. 70: 2245-2253 [Abstract] [Full Text]  
  • Campbell, B. J., Stein, J. L., Cary, S. C. (2003). Evidence of Chemolithoautotrophy in the Bacterial Community Associated with Alvinella pompejana, a Hydrothermal Vent Polychaete. Appl. Environ. Microbiol. 69: 5070-5078 [Abstract] [Full Text]  
  • Elsaied, H., Kimura, H., Naganuma, T. (2002). Molecular characterization and endosymbiotic localization of the gene encoding D-ribulose 1,5-bisphosphate carboxylase-oxygenase (RuBisCO) form II in the deep-sea vestimentiferan trophosome. Microbiology 148: 1947-1957 [Abstract] [Full Text]  
  • Elsaied, H., Naganuma, T. (2001). Phylogenetic Diversity of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Large-Subunit Genes from Deep-Sea Microorganisms. Appl. Environ. Microbiol. 67: 1751-1765 [Abstract] [Full Text]  
  • Baker, S. H., Jin, S., Aldrich, H. C., Howard, G. T., Shively, J. M. (1998). Insertion Mutation of the Form I cbbL Gene Encoding Ribulose Bisphosphate Carboxylase/Oxygenase (RuBisCO) in Thiobacillus neapolitanus Results in Expression of Form II RuBisCO, Loss of Carboxysomes, and an Increased CO2 Requirement for Growth. J. Bacteriol. 180: 4133-4139 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»