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J Bacteriol, April 1998, p. 1682-1690, Vol. 180, No. 7
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of the bvgR Locus of Bordetella pertussis

Tod J. Merkel,1,* Cassia Barros,1 and Scott Stibitz2

National Institute of Dental Research, National Institutes of Health,1 and Center for Biologics Evaluation and Research, U.S. Food and Drug Administration,2 Bethesda, Maryland 20892

Received 17 October 1997/Accepted 24 January 1998

Bordetella pertussis, the causative agent of whooping cough, produces a wide array of factors that are associated with its ability to cause disease. The expression and regulation of these virulence factors is dependent upon the bvg locus (originally designated the vir locus), which encodes two proteins: BvgA, a 23-kDa cytoplasmic protein, and BvgS, a 135-kDa transmembrane protein. It is proposed that BvgS responds to environmental signals and interacts with BvgA, a transcriptional regulator which upon modification by BvgS binds to specific promoters and activates transcription. An additional class of genes is repressed by the bvg locus. Expression of this class, the bvg-repressed genes (vrgs [for vir-repressed genes]), is reduced under conditions in which expression of the aforementioned bvg-activated virulence factors is maximal; this repression is dependent upon the presence of an intact bvgAS locus. We have previously identified a locus required for regulation of all of the known bvg-repressed genes in B. pertussis. This locus, designated bvgR, maps to a location immediately downstream of bvgAS. We have undertaken deletion and complementation studies, as well as sequence analysis, in order to identify the bvgR open reading frame and identify the cis-acting sequences required for regulated expression of bvgR. Studies utilizing transcriptional fusions of bvgR to the gene encoding alkaline phosphatase have demonstrated that bvgR is activated at the level of transcription and that this activation is dependent upon an intact bvgAS locus.

* Corresponding author. Mailing address: OIIB/NIDR/NIH, Building 30, Rm. 303, 30 Convent Dr. MSC 4350, Bethesda, MD 20892-4350. Phone: (301) 496-6060. Fax: (301) 402-0396. E-mail: merkel{at}yoda.nidr.nih.gov.




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