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J Bacteriol, April 1998, p. 1723-1728, Vol. 180, No. 7
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
The TolQRA Proteins Are Required for Membrane Insertion of
the Major Capsid Protein of the Filamentous Phage f1 during
Infection
Eva Marie
Click and
Robert E.
Webster*
Department of Biochemistry, Duke University
Medical Center, Durham, North Carolina 27710
Received 10 November 1997/Accepted 26 January 1998
Infection of Escherichia coli by the filamentous
bacteriophage f1 is initiated by interaction of the end of the phage
particle containing the gene III protein with the tip of the F
conjugative pilus. This is followed by the translocation of the phage
DNA into the cytoplasm and the insertion of the major phage capsid protein, pVIII, into the cytoplasmic membrane. DNA transfer requires the chromosomally encoded TolA, TolQ, and TolR cytoplasmic membrane proteins. By using radiolabeled phages, it can be shown that no pVIII
is inserted into the cytoplasmic membrane when the bacteria contain
null mutations in tolQ, -R and -A.
The rate of infection can be varied by using bacteria expressing
various mutant TolA proteins. Analysis of the infection process in
these strains demonstrates a direct correlation between the rate of
infection and the incorporation of infecting bacteriophage pVIII into
the cytoplasmic membrane.
*
Corresponding author. Mailing address: Department of
Biochemistry, Box 3711, Duke University Medical Center, Durham, NC
27710. Phone: (919) 683-3005. Fax: (919) 684-8885. E-mail:
webster{at}biochem.duke.edu.
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