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J Bacteriol, April 1998, p. 1766-1770, Vol. 180, No. 7
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Involvement of recF, recO, and recR Genes in UV-Radiation Mutagenesis of Escherichia coli

Ying-Hsiu Liu, Ann-Joy Cheng, and Tzu-chien V. Wang^*

Department of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan

Received 7 August 1997/Accepted 20 January 1998

The recF, recO, and recR genes were originally identified as those affecting the RecF pathway of recombination in Escherichia coli cells. Several lines of evidence suggest that the recF, recO, and recR genes function at the same step of recombination and postreplication repair. In this work, we report that null mutations in recF, recO, or recR greatly reduce UV-radiation mutagenesis (UVM) in an assay for reversion from a Trp (trpE65) to a Trp⁺ phenotypes. Introduction of the defective lexA51 mutation [lexA51(Def)] and/or UmuD' into recF, recO, and recR mutants failed to restore normal UVM in the mutants. On the other hand, the presence of recA2020, a suppressor mutation for recF, recO, and recR mutations, restored normal UVM in recF, recO, and recR mutants. These results indicate an involvement of the recF, recO, and recR genes and their products in UVM, possibly by affecting the third role of RecA in UVM.

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^* Corresponding author. Mailing address: Department of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan. Phone: 886-3-397-0147. Fax: 886-3-328-3031. E-mail: tcvwg{at}cguaplo.cgu.edu.tw.

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