This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Patel, J. B.
Right arrow Articles by Goldman, W. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Patel, J. B.
Right arrow Articles by Goldman, W. E.

 Previous Article  |  Next Article 

J Bacteriol, April 1998, p. 1786-1792, Vol. 180, No. 7
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Probing the Yeast Phase-Specific Expression of the CBP1 Gene in Histoplasma capsulatum

Jean Baldus Patel, Janet West Batanghari, and William E. Goldman*

Department of Molecular Microbiology, Washington University, St. Louis, Missouri 63110

Received 13 October 1997/Accepted 21 January 1998

Histoplasma capsulatum is a pathogenic fungus that exists in two distinct forms. The saprophytic mycelial phase inhabits moist soil environments; once inhaled, hyphae and conidia convert to a unicellular yeast phase that is capable of parasitizing macrophage phagolysosomes. Yeasts cultures, but not mycelial cultures, release large quantities of a calcium-binding protein (CBP) which may be important in calcium acquisition during intracellular parasitism. In this study, we show that the gene encoding CBP (CBP1) is transcriptionally regulated. To identify promoter sequences that are important for yeast phase-specific activity, we created a series of fusions between successively truncated CBP1 5' untranslated regulatory sequences and the Eschericha coli lacZ gene. The fusions were constructed on a telomeric shuttle plasmid that can replicate autonomously in the fungus. By assaying for beta -galactosidase activity from H. capsulatum transformants, we identified a 102-bp region that mediates promoter activation and yeast phase promoter activity. Base pair substitution analysis suggests that the sequences between 839 and 877 bp upstream of the start codon are the most important for this positive regulation.

* Corresponding author. Mailing address: Department of Molecular Microbiology, Campus Box 8230, Washington University School of Medicine, 660 S. Euclid Ave., St. Louis, MO 63110. Phone: (314) 362-2742. Fax: (314) 362-4879. E-mail: goldman{at}borcim.wustl.edu.




This article has been cited by other articles:

  • Monteiro, J. P., Clemons, K. V., Mirels, L. F., Coller, J. A. Jr, Wu, T. D., Shankar, J., Lopes, C. R., Stevens, D. A. (2009). Genomic DNA microarray comparison of gene expression patterns in Paracoccidioides brasiliensis mycelia and yeasts in vitro. Microbiology 155: 2795-2808 [Abstract] [Full Text]  
  • Bohse, M. L., Woods, J. P. (2007). RNA Interference-Mediated Silencing of the YPS3 Gene of Histoplasma capsulatum Reveals Virulence Defects. Infect. Immun. 75: 2811-2817 [Abstract] [Full Text]  
  • Bohse, M. L., Woods, J. P. (2007). Expression and Interstrain Variability of the YPS3 Gene of Histoplasma capsulatum. Eukaryot Cell 6: 609-615 [Abstract] [Full Text]  
  • Gebhart, D., Bahrami, A. K., Sil, A. (2006). Identification of a Copper-Inducible Promoter for Use in Ectopic Expression in the Fungal Pathogen Histoplasma capsulatum. Eukaryot Cell 5: 935-944 [Abstract] [Full Text]  
  • Vanittanakom, N., Cooper, C. R. Jr., Fisher, M. C., Sirisanthana, T. (2006). Penicillium marneffei Infection and Recent Advances in the Epidemiology and Molecular Biology Aspects. Clin. Microbiol. Rev. 19: 95-110 [Abstract] [Full Text]  
  • Bohse, M. L., Woods, J. P. (2005). Surface Localization of the Yps3p Protein of Histoplasma capsulatum. Eukaryot Cell 4: 685-693 [Abstract] [Full Text]  
  • Rooney, P. J., Klein, B. S. (2004). Sequence Elements Necessary for Transcriptional Activation of BAD1 in the Yeast Phase of Blastomyces dermatitidis. Eukaryot Cell 3: 785-794 [Abstract] [Full Text]  
  • Hwang, L., Hocking-Murray, D., Bahrami, A. K., Andersson, M., Rine, J., Sil, A. (2003). Identifying Phase-specific Genes in the Fungal Pathogen Histoplasma capsulatum Using a Genomic Shotgun Microarray. Mol. Biol. Cell 14: 2314-2326 [Abstract] [Full Text]  
  • Magee, P. T., Gale, C., Berman, J., Davis, D. (2003). Molecular Genetic and Genomic Approaches to the Study of Medically Important Fungi. Infect. Immun. 71: 2299-2309 [Full Text]  
  • Sullivan, T. D., Rooney, P. J., Klein, B. S. (2002). Agrobacterium tumefaciens Integrates Transfer DNA into Single Chromosomal Sites of Dimorphic Fungi and Yields Homokaryotic Progeny from Multinucleate Yeast. Eukaryot Cell 1: 895-905 [Abstract] [Full Text]  
  • Ignatov, A., Keath, E. J. (2002). Gel Shift Assay of Nuclear Extracts from Histoplasma capsulatum Demonstrates the Presence of Several DNA Binding Proteins. Infect. Immun. 70: 2238-2241 [Abstract] [Full Text]  
  • Kugler, S., Sebghati, T. S., Eissenberg, L. G., Goldman, W. E. (2000). Phenotypic variation and intracellular parasitism by Histoplasmacapsulatum. Proc. Natl. Acad. Sci. USA 97: 8794-8798 [Abstract] [Full Text]  
  • Woods, J. P., Retallack, D. M., Heinecke, E. L., Goldman, W. E. (1998). Rare Homologous Gene Targeting in Histoplasma capsulatum: Disruption of the URA5Hc Gene by Allelic Replacement. J. Bacteriol. 180: 5135-5143 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»