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J Bacteriol, April 1998, p. 1955-1958, Vol. 180, No. 7
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Frequency of Pilin Antigenic Variation in Neisseria gonorrhoeae

Carla D. Serkin and H. Steven Seifert*

Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, Illinois 60611

Received 22 September 1997/Accepted 26 January 1998

Variation of the pilus of Neisseria gonorrhoeae occurs by the recombination of silent pilin DNA sequences into the pilin expression locus. We have developed a quantitative, competitive reverse transcription-PCR assay which measures the frequency of pilin antigenic variation independently of changes in gonococcal colony morphology and have determined this frequency within a gonococcal population. We have also studied the frequency of antigenic variation during growth and have concluded that growth does not dramatically influence the frequency of pilin antigenic variation, although a reproducible, twofold increase is observed upon the transition into late log/stationary phase.

* Corresponding author. Mailing address: Department of Microbiology-Immunology, Northwestern University Medical School, 303 East Chicago Ave., Chicago, IL, 60611. Phone: (312) 503-9788. Fax: (312) 503-1339. E-mail: h-seifert{at}nwu.edu.




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