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J Bacteriol, April 1998, p. 1962-1964, Vol. 180, No. 7
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Proton Gradient-Driven Nickel Uptake by Vacuolar Membrane Vesicles of Saccharomyces cerevisiae

Ken Nishimura, Kazuei Igarashi, and Yoshimi Kakinuma^*

Faculty of Pharmaceutical Sciences, Chiba University, Inage-ku, Chiba 263, Japan

Received 18 November 1997/Accepted 3 February 1998

A vacuolar H⁺-ATPase-negative mutant of Saccharomyces cerevisiae was highly sensitive to nickel ion. Accumulation of nickel ion in the cells of this mutant of less than 60% of the value for the parent strain arrested growth, suggesting a role for this ATPase in sequestering nickel ion into vacuoles. An artificially imposed pH gradient (interior acid) induced transient nickel ion uptake by vacuolar membrane vesicles, which was inhibited by collapse of the pH difference but not of the membrane potential. Nickel ion transport into vacuoles in a pH gradient-dependent manner is thus important for its detoxification in yeast.

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^* Corresponding author. Mailing address: Faculty of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263, Japan. Phone: 81-43-290-2898. Fax: 81-43-290-2900. E-mail: yoshimi{at}athenaeum.p.chiba-u.ac.jp.

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